Polr2b polymerase (RNA) II (DNA directed) polypeptide B [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Polr2bprovided by MGI
Official Full Name: polymerase (RNA) II (DNA directed) polypeptide Bprovided by MGI
Primary source: MGI:MGI:2388280
See related: Ensembl:ENSMUSG00000029250 AllianceGenome:MGI:2388280
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rpb2; Pol2rb; Rpb140
Summary: Enables chromatin binding activity. Predicted to be involved in transcription by RNA polymerase II. Predicted to be located in nucleoplasm. Predicted to be part of RNA polymerase II, core complex. Predicted to colocalize with chromosome, telomeric region. Is expressed in several structures, including alimentary system; brain; integumental system; respiratory system; and sensory organ. Orthologous to human POLR2B (RNA polymerase II subunit B). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 29.1), CNS E14 (RPKM 19.8) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 C3.3; 5 41.66 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (77458331..77497175)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (77310484..77349328)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TANGO6 regulates cell proliferation via COPI vesicle-mediated RPB2 nuclear entry.
Title: TANGO6 regulates cell proliferation via COPI vesicle-mediated RPB2 nuclear entry.
For mouse embryonic stem cells with induced neural differentiation, all strategies used in parallel indicate RpII to be expressed in a stable manner.
Title: Reference genes in the developing murine brain and in differentiating embryonic stem cells.
NF-Y recruits RNAPII and general transcription factors onto various CCAAT box-containing promoters to permit strong transcriptional activation independently of histone modifications
Title: NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Igfbp7 (e-PCR), detects polymorphism
- UniSTS:498744

D11Got68 (e-PCR)
- UniSTS:115593

Polr2b (e-PCR), detects polymorphism
- UniSTS:547288

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed 5'-3' RNA polymerase activity	ISO Inferred from Sequence Orthology more info
contributes_to RNA polymerase II activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA-dependent RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables RNA-dependent RNA polymerase activity	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables ribonucleoside binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of RNA polymerase II, core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II, core complex	ISO Inferred from Sequence Orthology more info
part_of RNA polymerase II, core complex	ISS Inferred from Sequence or Structural Similarity more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
colocalizes_with chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA-directed RNA polymerase II subunit RPB2

Names: 3'-5' exoribonuclease; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II subunit 2; RNA polymerase II subunit B2; RNA-directed RNA polymerase II subunit RPB2

NP_722493.2

EC 2.7.7.48

EC 2.7.7.6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_153798.2 → NP_722493.2 DNA-directed RNA polymerase II subunit RPB2

See identical proteins and their annotated locations for NP_722493.2

Status: VALIDATED

Source sequence(s)

AA501042, AK039766, BQ177004, CF155991, CF553167, CN690754

Consensus CDS

CCDS51528.1

UniProtKB/Swiss-Prot

Q8CFI7

Related

ENSMUSP00000031167.6, ENSMUST00000031167.6

Conserved Domains (4) summary

PRK08565
Location:27 → 1172

PRK08565; DNA-directed RNA polymerase subunit B; Provisional

cd00653
Location:39 → 1172

RNA_pol_B_RPB2; RNA polymerase beta subunit. RNA polymerases catalyse the DNA dependent polymerization of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). Each RNA polymerase ...

pfam04566
Location:567 → 629

RNA_pol_Rpb2_4; RNA polymerase Rpb2, domain 4

pfam04567
Location:653 → 700

RNA_pol_Rpb2_5; RNA polymerase Rpb2, domain 5