Capns1 calpain, small subunit 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Capns1provided by MGI
Official Full Name: calpain, small subunit 1provided by MGI
Primary source: MGI:MGI:88266
See related: Ensembl:ENSMUSG00000001794 AllianceGenome:MGI:88266
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cdps; Css1; Capa4; Capn4; Capa-4; D7Ertd146e
Summary: Enables calcium-dependent cysteine-type endopeptidase activity. Predicted to be involved in protein catabolic process. Predicted to be located in cytosol and membrane. Predicted to be part of calpain complex. Is expressed in medulla oblongata basal plate mantle layer; nucleus pulposus; pons mantle layer; and ventral grey horn. Orthologous to human CAPNS1 (calpain small subunit 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in adrenal adult (RPKM 274.2), mammary gland adult (RPKM 214.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 B1; 7 17.31 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (29886359..29898308, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (30186934..30198883, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Macrophage CAPN4 regulates CVB3-induced cardiac inflammation and injury by promoting NLRP3 inflammasome activation and phenotypic transformation to the inflammatory subtype.
Title: Macrophage CAPN4 regulates CVB3-induced cardiac inflammation and injury by promoting NLRP3 inflammasome activation and phenotypic transformation to the inflammatory subtype.
Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis.
Title: Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis.
Identification of Crucial Genes and Key Functions in Type 2 Diabetic Hearts by Bioinformatic Analysis.
Title: Identification of Crucial Genes and Key Functions in Type 2 Diabetic Hearts by Bioinformatic Analysis.
Capn4 aggravates angiotensin II-induced cardiac hypertrophy by activating the IGF-AKT signalling pathway.
Title: Capn4 aggravates angiotensin II-induced cardiac hypertrophy by activating the IGF-AKT signalling pathway.
Calpain activation mediates microgravity-induced myocardial abnormalities in mice via p38 and ERK1/2 MAPK pathways.
Title: Calpain activation mediates microgravity-induced myocardial abnormalities in mice via p38 and ERK1/2 MAPK pathways.
deletion of Capns1 elevated the protein levels of beta-catenin in endothelial cells of Capns1-KO mice and constitutive activity of calpain 2 suppressed beta-catenin protein expression in cultured endothelial cells
Title: Selective deletion of endothelial cell calpain in mice reduces diabetic cardiomyopathy by improving angiogenesis.
Mitochondrial calpain-1 disrupts ATP synthase, leading to mitochondrial reactive oxygen species generation, which promotes proinflammatory response and myocardial dysfunction during endotoxemia.
Title: Deletion of capn4 Protects the Heart Against Endotoxemic Injury by Preventing ATP Synthase Disruption and Inhibiting Mitochondrial Superoxide Generation.
Calpain in osteoblasts regulates cell proliferation through its regulation of p27Kip1. Osteoblast-specific capns1 knockout mice developed a high-fat diet-induced excess body weight gain and impaired glucose tolerance.
Title: Overview of calpain-mediated regulation of bone and fat mass in osteoblasts.
effects of Capn4 deletion may be mediated through prevention of IkappaB degradation and NF-kappaB activation, resulting in inhibition of inflammatory responses.
Title: Deficiency of Capn4 gene inhibits nuclear factor-κB (NF-κB) protein signaling/inflammation and reduces remodeling after myocardial infarction.
Calpain 4 is essential for the tyrosine phosphorylation of galectin-3, and its ultimate secretion from the cell, and its secretion probably interferes with the production of traction forces.
Title: Galectin-3 secretion and tyrosine phosphorylation is dependent on the calpain small subunit, Calpain 4.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D7Ertd146e (e-PCR), detects polymorphism
- UniSTS:167503

NoName (e-PCR)
- UniSTS:225133

AF058298 (e-PCR)
- UniSTS:159621

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium-dependent cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to calcium-dependent cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of calpain complex	IBA Inferred from Biological aspect of Ancestor more info
part_of calpain complex	ISO Inferred from Sequence Orthology more info
part_of calpain complex	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: calpain small subunit 1

Names: CANP small subunit; calcium-activated neutral proteinase small subunit; calcium-dependent protease small subunit 1; calpain 4; calpain regulatory subunit

NP_033925.2

EC 3.4.22.17

XP_006539551.1

EC 3.4.22.17

XP_011248722.1

EC 3.4.22.17

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009795.4 → NP_033925.2 calpain small subunit 1

See identical proteins and their annotated locations for NP_033925.2

Status: VALIDATED

Source sequence(s)

BC090988, BU700497, BY133208, BY296628

Consensus CDS

CCDS21082.1

UniProtKB/Swiss-Prot

A0A0R4IZW8, D3YW48, O88456, Q3V0X5, Q5BKQ2, Q8CEI2, Q8VEK4, Q9R1C5

Related

ENSMUSP00000117951.3, ENSMUST00000126116.3

Conserved Domains (1) summary

cd16188
Location:100 → 268

EFh_PEF_CPNS1_2; Penta-EF hand, calcium binding motifs, found in calcium-dependent protease small subunit CAPNS1 and CAPNS2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

29886359..29898308 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006539488.3 → XP_006539551.1 calpain small subunit 1 isoform X1

See identical proteins and their annotated locations for XP_006539551.1

UniProtKB/Swiss-Prot

A0A0R4IZW8, D3YW48, O88456, Q3V0X5, Q5BKQ2, Q8CEI2, Q8VEK4, Q9R1C5

Related

ENSMUSP00000001845.6, ENSMUST00000001845.13

Conserved Domains (1) summary

cd16188
Location:100 → 268

EFh_PEF_CPNS1_2; Penta-EF hand, calcium binding motifs, found in calcium-dependent protease small subunit CAPNS1 and CAPNS2
XM_011250420.1 → XP_011248722.1 calpain small subunit 1 isoform X2

Conserved Domains (1) summary

cd16188
Location:1 → 156

EFh_PEF_CPNS1_2; Penta-EF hand, calcium binding motifs, found in calcium-dependent protease small subunit CAPNS1 and CAPNS2