Epha4 Eph receptor A4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Epha4provided by MGI
Official Full Name: Eph receptor A4provided by MGI
Primary source: MGI:MGI:98277
See related: Ensembl:ENSMUSG00000026235 AllianceGenome:MGI:98277
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: rb; Sek; Cek8; Hek8; Sek1; Tyro1; 2900005C20Rik
Summary: Enables GPI-linked ephrin receptor activity; identical protein binding activity; and transmembrane-ephrin receptor activity. Involved in several processes, including axon development; positive regulation of protein kinase activity; and regulation of neuron projection development. Acts upstream of or within several processes, including adult walking behavior; nephric duct morphogenesis; and nervous system development. Located in several cellular components, including axon; dendrite; and early endosome membrane. Is active in Schaffer collateral - CA1 synapse; glutamatergic synapse; and synaptic membrane. Is expressed in several structures, including alimentary system; central nervous system; embryo mesenchyme; genitourinary system; and sensory organ. Orthologous to human EPHA4 (EPH receptor A4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cortex adult (RPKM 17.3), whole brain E14.5 (RPKM 14.7) and 13 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 C4; 1 39.55 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (77343819..77491763, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (77367182..77515107, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrity.
Title: Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrity.
Transcriptional regulation of the mouse EphA4, Ephrin-B2 and Ephrin-A3 genes by the circadian clock machinery.
Title: Transcriptional regulation of the mouse EphA4, Ephrin-B2 and Ephrin-A3 genes by the circadian clock machinery.
Mechanistic and therapeutic implications of EphA-4 receptor tyrosine kinase in the pathogenesis of Alzheimer's disease.
Title: Mechanistic and therapeutic implications of EphA-4 receptor tyrosine kinase in the pathogenesis of Alzheimer's disease.
Altered thymocyte development observed in EphA4-deficient mice courses with changes in both thymic epithelial and extracellular matrix organization.
Title: Altered thymocyte development observed in EphA4-deficient mice courses with changes in both thymic epithelial and extracellular matrix organization.
The Eph receptor A4 plays a role in demyelination and depression-related behavior.
Title: The Eph receptor A4 plays a role in demyelination and depression-related behavior.
Dissecting indirect genetic effects from peers in laboratory mice.
Title: Dissecting indirect genetic effects from peers in laboratory mice.
Human urine-derived stem cell-derived exosomal miR-21-5p promotes neurogenesis to attenuate Rett syndrome via the EPha4/TEK axis.
Title: Human urine-derived stem cell-derived exosomal miR-21-5p promotes neurogenesis to attenuate Rett syndrome via the EPha4/TEK axis.
The EphA4 Signaling is Anti-catabolic in Synoviocytes but Pro-anabolic in Articular Chondrocytes.
Title: The EphA4 Signaling is Anti-catabolic in Synoviocytes but Pro-anabolic in Articular Chondrocytes.
EphA4 Is Required for Neural Circuits Controlling Skilled Reaching.
Title: EphA4 Is Required for Neural Circuits Controlling Skilled Reaching.
Inducible EphA4 knockout causes motor deficits in young mice and is not protective in the SOD1(G93A) mouse model of ALS.
Title: Inducible EphA4 knockout causes motor deficits in young mice and is not protective in the SOD1(G93A) mouse model of ALS.

Submit: New GeneRIF Correction See all GeneRIFs (112)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Endonuclease-mediated (3)
Gene trapped (1) 1 citation
Spontaneous (2)
Targeted (16) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Epha4 (e-PCR), detects polymorphism
- UniSTS:495882

NoName (e-PCR)
- UniSTS:548163

PMC23772P1 (e-PCR)
- UniSTS:272208

NoName (e-PCR)
- UniSTS:548480

Epha4 (e-PCR), detects polymorphism
- UniSTS:547980

NoName (e-PCR)
- UniSTS:234159

D1Mit332 (e-PCR), detects polymorphism
- UniSTS:128690

X65138 (e-PCR)
- UniSTS:163254

Epha4 (e-PCR), detects polymorphism
- UniSTS:527335

AI385584 (e-PCR)
- UniSTS:178416

fk56e12.x1 (e-PCR)
- UniSTS:201209

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DH domain binding	ISO Inferred from Sequence Orthology more info
enables DH domain binding	ISS Inferred from Sequence or Structural Similarity more info
enables GPI-linked ephrin receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables PH domain binding	IEA Inferred from Electronic Annotation more info
enables PH domain binding	ISO Inferred from Sequence Orthology more info
enables ephrin receptor binding	IEA Inferred from Electronic Annotation more info
enables ephrin receptor binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein tyrosine kinase binding	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables transmembrane receptor protein tyrosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane-ephrin receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables transmembrane-ephrin receptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in adherens junction organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within adult walking behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cellular response to amyloid-beta	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cochlea development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in corticospinal tract morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ephrin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in ephrin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in ephrin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in fasciculation of motor neuron axon	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fasciculation of sensory neuron axon	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glial cell migration	IEA Inferred from Electronic Annotation more info
involved_in glial cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within innervation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in motor neuron axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of ERK1 and ERK2 cascade	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of axon regeneration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of axon regeneration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cellular response to hypoxia	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of cellular response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of epithelial to mesenchymal transition	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of epithelial to mesenchymal transition	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of long-term synaptic potentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron projection development	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of proteolysis involved in protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of proteolysis involved in protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nephric duct morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron projection fasciculation	IDA Inferred from Direct Assay more info	PubMed
involved_in neuron projection guidance	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-tyrosine phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of JUN kinase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of Rho guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of Rho guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of amyloid-beta formation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of amyloid-beta formation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendrite morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein tyrosine kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein stabilization	IEA Inferred from Electronic Annotation more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
involved_in regulation of GTPase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of astrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of dendritic spine morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of modification of synaptic structure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of modification of synaptic structure	ISO Inferred from Sequence Orthology more info
involved_in regulation of synapse pruning	IEA Inferred from Electronic Annotation more info
involved_in regulation of synapse pruning	ISO Inferred from Sequence Orthology more info
involved_in synapse pruning	EXP Inferred from Experiment more info	PubMed
involved_in synapse pruning	IDA Inferred from Direct Assay more info	PubMed
involved_in synapse pruning	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IEP Inferred from Expression Pattern more info	PubMed
located_in adherens junction	IEA Inferred from Electronic Annotation more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon terminus	IEA Inferred from Electronic Annotation more info
located_in axon terminus	ISO Inferred from Sequence Orthology more info
located_in axonal growth cone	IEA Inferred from Electronic Annotation more info
located_in axonal growth cone	ISO Inferred from Sequence Orthology more info
located_in cell body	ISO Inferred from Sequence Orthology more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cell surface	IEA Inferred from Electronic Annotation more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dendritic shaft	IEA Inferred from Electronic Annotation more info
located_in dendritic shaft	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	IEA Inferred from Electronic Annotation more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in filopodium	IEA Inferred from Electronic Annotation more info
located_in filopodium	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in neuromuscular junction	IEA Inferred from Electronic Annotation more info
located_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
located_in perikaryon	IEA Inferred from Electronic Annotation more info
located_in perikaryon	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic membrane	IEP Inferred from Expression Pattern more info	PubMed
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IEP Inferred from Expression Pattern more info	PubMed

General protein information

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Preferred Names: ephrin type-A receptor 4

Names: tyrosine-protein kinase receptor MPK-3; tyrosine-protein kinase receptor SEK-1

NP_031962.2

EC 2.7.10.1

XP_006496470.1

EC 2.7.10.1

XP_006496471.1

EC 2.7.10.1

XP_036015237.1

EC 2.7.10.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007936.3 → NP_031962.2 ephrin type-A receptor 4 precursor

See identical proteins and their annotated locations for NP_031962.2

Status: VALIDATED

Source sequence(s)

AC164875, AK147698, X65138

Consensus CDS

CCDS35627.1

UniProtKB/Swiss-Prot

Q03137, Q80VZ2

UniProtKB/TrEMBL

Q99KA8

Related

ENSMUSP00000027451.7, ENSMUST00000027451.13

Conserved Domains (7) summary

cd09545
Location:911 → 981

SAM_EPH-A4; SAM domain of EPH-A4 subfamily of tyrosine kinase receptors

smart00454
Location:915 → 975

SAM; Sterile alpha motif

cd10482
Location:30 → 203

EphR_LBD_A4; Ligand Binding Domain of Ephrin type-A Receptor 4

cd00063
Location:441 → 532

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd05066
Location:616 → 882

PTKc_EphR_A; Catalytic domain of the Protein Tyrosine Kinases, Class EphA Ephrin Receptors

pfam07714
Location:621 → 878

Pkinase_Tyr; Protein tyrosine kinase

pfam14575
Location:562 → 616

EphA2_TM; Ephrin type-A receptor 2 transmembrane domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

77343819..77491763 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006496407.4 → XP_006496470.1 ephrin type-A receptor 4 isoform X1

UniProtKB/TrEMBL

Q99KA8

Conserved Domains (5) summary

cd10482
Location:30 → 203

EphR_LBD_A4; Ligand Binding Domain of Ephrin type-A Receptor 4

cd00063
Location:441 → 532

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd05066
Location:616 → 882

PTKc_EphR_A; Catalytic domain of the Protein Tyrosine Kinases, Class EphA Ephrin Receptors

pfam14575
Location:562 → 616

EphA2_TM; Ephrin type-A receptor 2 transmembrane domain

cl15755
Location:911 → 949

SAM_superfamily; SAM (Sterile alpha motif )
XM_006496408.3 → XP_006496471.1 ephrin type-A receptor 4 isoform X2

UniProtKB/TrEMBL

Q99KA8

Conserved Domains (5) summary

cd09545
Location:860 → 930

SAM_EPH-A4; SAM domain of EPH-A4 subfamily of tyrosine kinase receptors

cd10473
Location:3 → 152

EphR_LBD_A; Ligand Binding Domain of Ephrin type-A Receptors

cd00063
Location:390 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd05066
Location:565 → 831

PTKc_EphR_A; Catalytic domain of the Protein Tyrosine Kinases, Class EphA Ephrin Receptors

pfam14575
Location:511 → 565

EphA2_TM; Ephrin type-A receptor 2 transmembrane domain
XM_036159344.1 → XP_036015237.1 ephrin type-A receptor 4 isoform X3

UniProtKB/TrEMBL

Q99KA8

Conserved Domains (5) summary

cd10473
Location:3 → 152

EphR_LBD_A; Ligand Binding Domain of Ephrin type-A Receptors

cd00063
Location:390 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd05066
Location:565 → 831

PTKc_EphR_A; Catalytic domain of the Protein Tyrosine Kinases, Class EphA Ephrin Receptors

pfam14575
Location:511 → 567

EphA2_TM; Ephrin type-A receptor 2 transmembrane domain

cl15755
Location:860 → 898

SAM_superfamily; SAM (Sterile alpha motif )