Ago2 argonaute RISC catalytic subunit 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ago2provided by MGI
Official Full Name: argonaute RISC catalytic subunit 2provided by MGI
Primary source: MGI:MGI:2446632
See related: Ensembl:ENSMUSG00000036698 AllianceGenome:MGI:2446632
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Eif2c2; Gerp95; Gm10365; mKIAA4215; 1110029L17Rik; 2310051F07Rik
Summary: Enables RNA endonuclease activity; mRNA binding activity; and miRNA binding activity. Involved in positive regulation of mRNA catabolic process and pre-miRNA processing. Acts upstream of or within RNA metabolic process; positive regulation of gene expression; and post-embryonic development. Located in several cellular components, including P-body; cytosol; and dendrite. Part of RISC complex and RISC-loading complex. Is expressed in several structures, including central nervous system; early embryo; lung; sensory organ; and tooth. Human ortholog(s) of this gene implicated in alcohol dependence and glaucoma. Orthologous to human AGO2 (argonaute RISC catalytic component 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 19.6), spleen adult (RPKM 13.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 D3; 15 33.92 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (72967693..73056777, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (73101625..73184947, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sex-dependent phosphorylation of Argonaute 2 reduces the mitochondrial translocation of miR-181c and induces cardioprotection in females.
Title: Sex-dependent phosphorylation of Argonaute 2 reduces the mitochondrial translocation of miR-181c and induces cardioprotection in females.
Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction.
Title: Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction.
Argonaute 2 restored erectile function and corpus cavernosum mitochondrial function by reducing apoptosis in a mouse model of cavernous nerve injury.
Title: Argonaute 2 restored erectile function and corpus cavernosum mitochondrial function by reducing apoptosis in a mouse model of cavernous nerve injury.
AGO2 silences mobile transposons in the nucleus of quiescent cells.
Title: AGO2 silences mobile transposons in the nucleus of quiescent cells.
Pycard deficiency inhibits microRNA maturation and prevents neointima formation by promoting chaperone-mediated autophagic degradation of AGO2/argonaute 2 in adipose tissue.
Title: Pycard deficiency inhibits microRNA maturation and prevents neointima formation by promoting chaperone-mediated autophagic degradation of AGO2/argonaute 2 in adipose tissue.
hsa_circ_0129047 Upregulates LYVE1 to Inhibit Hepatocellular Carcinoma Progression by Sponging miR-492.
Title: hsa_circ_0129047 Upregulates LYVE1 to Inhibit Hepatocellular Carcinoma Progression by Sponging miR-492.
Long non-coding RNA PFI inhibits apoptosis of alveolar epithelial cells to alleviate lung injury via miR-328-3p/Creb1 axis.
Title: Long non-coding RNA PFI inhibits apoptosis of alveolar epithelial cells to alleviate lung injury via miR-328-3p/Creb1 axis.
LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways.
Title: LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways.
Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage in antibody diversification by activation-induced cytidine deaminase.
Title: Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage in antibody diversification by activation-induced cytidine deaminase.
Argonaute 2 Restores Erectile Function by Enhancing Angiogenesis and Reducing Reactive Oxygen Species Production in Streptozotocin (STZ)-Induced Type-1 Diabetic Mice.
Title: Argonaute 2 Restores Erectile Function by Enhancing Angiogenesis and Reducing Reactive Oxygen Species Production in Streptozotocin (STZ)-Induced Type-1 Diabetic Mice.

Submit: New GeneRIF Correction See all GeneRIFs (63)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Gene trapped (1) 1 citation
Targeted (10) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237320

Eif2c2 (e-PCR), detects polymorphism
- UniSTS:471827

AW546247 (e-PCR)
- UniSTS:180769

RH135997 (e-PCR)
- UniSTS:209993

D15Mit197 (e-PCR), detects polymorphism
- UniSTS:127563

BG378661 (e-PCR)
- UniSTS:245094

Eif2c2 (e-PCR), detects polymorphism
- UniSTS:479102

RH125371 (e-PCR)
- UniSTS:162193

Eif2c2 (e-PCR), detects polymorphism
- UniSTS:479157

42.MMHAP37FRF12.seq (e-PCR)
- UniSTS:186315

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables Hsp70 protein binding	ISO Inferred from Sequence Orthology more info
enables Hsp90 protein binding	ISO Inferred from Sequence Orthology more info
enables RISC complex binding	ISO Inferred from Sequence Orthology more info
enables RNA 7-methylguanosine cap binding	ISO Inferred from Sequence Orthology more info
enables RNA 7-methylguanosine cap binding	ISS Inferred from Sequence or Structural Similarity more info
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II complex binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II complex binding	ISO Inferred from Sequence Orthology more info
enables core promoter sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables core promoter sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables double-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving miRNA-paired mRNA	IEA Inferred from Electronic Annotation more info
enables endoribonuclease activity, cleaving miRNA-paired mRNA	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving siRNA-paired mRNA	ISO Inferred from Sequence Orthology more info
enables endoribonuclease activity, cleaving siRNA-paired mRNA	ISS Inferred from Sequence or Structural Similarity more info
enables endoribonuclease activity, cleaving siRNA-paired mRNA	TAS Traceable Author Statement more info
enables mRNA 3'-UTR AU-rich region binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR AU-rich region binding	ISO Inferred from Sequence Orthology more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA cap binding	ISO Inferred from Sequence Orthology more info
enables mRNA cap binding	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables miRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables miRNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
contributes_to siRNA binding	ISO Inferred from Sequence Orthology more info
enables siRNA binding	ISO Inferred from Sequence Orthology more info
enables siRNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in P-body assembly	IEA Inferred from Electronic Annotation more info
involved_in P-body assembly	ISO Inferred from Sequence Orthology more info
involved_in RISC complex assembly	ISO Inferred from Sequence Orthology more info
involved_in RNA secondary structure unwinding	IEA Inferred from Electronic Annotation more info
involved_in RNA secondary structure unwinding	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within miRNA metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISS Inferred from Sequence or Structural Similarity more info
involved_in miRNA-mediated gene silencing by mRNA destabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in miRNA-mediated gene silencing by mRNA destabilization	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by mRNA destabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of amyloid precursor protein biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translational initiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translational initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of post-transcriptional gene silencing by RNA	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of trophoblast cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of trophoblast cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within post-embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in pre-miRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in pre-miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in pre-miRNA processing	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of mRNA stability	ISO Inferred from Sequence Orthology more info
involved_in regulation of synapse maturation	IEA Inferred from Electronic Annotation more info
involved_in regulation of synapse maturation	ISO Inferred from Sequence Orthology more info
involved_in regulatory ncRNA-mediated gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in siRNA processing	ISO Inferred from Sequence Orthology more info
involved_in siRNA-mediated gene silencing by mRNA destabilization	IEA Inferred from Electronic Annotation more info
involved_in siRNA-mediated gene silencing by mRNA destabilization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in P granule	ISO Inferred from Sequence Orthology more info
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	ISO Inferred from Sequence Orthology more info
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	ISO Inferred from Sequence Orthology more info
part_of RISC complex	ISS Inferred from Sequence or Structural Similarity more info
part_of RISC-loading complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC-loading complex	IPI Inferred from Physical Interaction more info	PubMed
part_of RISC-loading complex	ISO Inferred from Sequence Orthology more info
part_of RISC-loading complex	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatoid body	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info	PubMed
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic stress granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in extracellular exosome	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in pi-body	ISO Inferred from Sequence Orthology more info
located_in postsynapse	IEA Inferred from Electronic Annotation more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein argonaute-2

Names: Piwi/Argonaute family protein meIF2C2; argonaute 2; argonaute RISC catalytic component 2; eukaryotic translation initiation factor 2C, 2

NP_694818.3

EC 3.1.26.n2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_153178.5 → NP_694818.3 protein argonaute-2

See identical proteins and their annotated locations for NP_694818.3

Status: VALIDATED

Source sequence(s)

AC118640, AC161581

Consensus CDS

CCDS37098.1

UniProtKB/Swiss-Prot

A1A563, Q4VAB3, Q571J6, Q8CJG0

UniProtKB/TrEMBL

Q8CGT9

Related

ENSMUSP00000042207.10, ENSMUST00000044113.12

Conserved Domains (2) summary

PLN03202
Location:7 → 859

PLN03202; protein argonaute; Provisional

cd04657
Location:393 → 818

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...