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Series GSE82113 Query DataSets for GSE82113
Status Public on Feb 08, 2017
Title Mistimed sleep disrupts circadian regulation of the human blood transcriptome - Heterozygotes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary 4 healthy volunteers without sleep disorders were resident in an environmental scheduling facility and participated in a forced-desynchrony protocol, in which the sleep-wake cycle and the associated fasting-feeding cycle is scheduled to a 28-hour period, of which one third (i.e. 9h 20min) is scheduled for sleep. Under these conditions, during which light levels in the waking episode are kept low and sleep is scheduled in darkness, the phase of the melatonin rhythm occurred at approximately the same clock time during the first (D1) and fourth (D4) 28-h cycle and there were no major changes in either the amplitude or the waveform of this rhythm. During D1 and D4 7 blood samples were taken, RNA was extracted from leukocytes, labelled and hybridised to human whole-genome microarrays
 
Overall design A total of 23 samples comprising 4 human subjects, for which 14 samples across multiple time-points/sleep condition were collected.
 
Contributor(s) Moller-Levet C, Archer S, Bucca G, Laing E, Slak A, Kabiljo R, Lo J, Santhi N, von Schantz M, Smith CP, Dijk DJ
Citation(s) 28218891
Submission date Jun 01, 2016
Last update date May 11, 2017
Contact name Emma Laing
E-mail(s) e.laing@surrey.ac.uk
Organization name University of Surrey
Street address Stag Hill
City Guildford
ZIP/Postal code GU22 7XH
Country United Kingdom
 
Platforms (1)
GPL15331 Agilent-026817 University_Surrey_HumanSleep_44k_v1
Samples (23)
GSM2183625 BloodAtTimepoint5,followingSleepInPhaseWithMelatoninForSubjectBB0008
GSM2183626 BloodAtTimepoint6,followingSleepInPhaseWithMelatoninForSubjectBB0008
GSM2183627 BloodAtTimepoint7,followingSleepInPhaseWithMelatoninForSubjectBB0008
Relations
BioProject PRJNA324077

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82113_RAW.tar 48.3 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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