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| Status |
Public on May 30, 2014 |
| Title |
Prefrontal cortex microRNA expression profiling in major depression |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To gain insight into the potential role of miRNA in major depressive disorder, in this study we assessed global expression of miRNAs in the ventrolateral prefrontal cortex (PFC) of depressed individuals in comparison to psychiatrically healthy controls. Our screening pointed to miR-1202, a miRNA specific to primates and enriched in the human brain. We validated our findings using quantitative real-time PCR and identified target genes of the differentially expressed miRNAs using bioinformatics analysis. MiR-1202 interacts with and regulates the expression of the metabotropic glutamate receptor 4 (GRM4) gene, and it responds to antidepressant treatment. These results suggest that miR-1202 is associated with the pathophysiology of depression, and is a potential target for novel antidepressant treatments.
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| Overall design |
We assessed the global expression pattern of miRNAs in the ventrolateral PFC of depressed individuals (n = 14) in comparison with psychiatrically healthy controls (n = 11) using the Agilent human miRNA microarrays. Statistical analysis after multiple test correction identified miR-1202 as the most significantly dysregulated miRNA, with levels down-regulated in depressed brains. We validated the microarray miR-1202 findings by qRT-PCR using TaqMan probes, and found consistent results across the two expression analysis methods.
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| Contributor(s) |
Lopez JP, Lim R, Pavlidis P, Turecki G |
| Citation(s) |
24908571 |
| Submission date |
May 29, 2014 |
| Last update date |
Aug 13, 2014 |
| Contact name |
Paul Pavlidis |
| E-mail(s) |
paul@chibi.ubc.ca
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| Organization name |
University of British Columbia
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| Department |
Centre for High-Throughput Biology / Psychiatry
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| Street address |
177 Michael Smith Laboratories 2185 East Mall
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| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
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| Platforms (1) |
| GPL18743 |
Agilent-021827 Human miRNA Microarray [miRNA_107_Sep09] |
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| Samples (25)
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| GSM1400874 |
Control, S4.BA44.1.C |
| GSM1400875 |
Control, S6.BA44.1.C |
| GSM1400876 |
Control, S56.BA44.4.C |
| GSM1400877 |
Control, S12.BA44.1.C |
| GSM1400878 |
Control, S58.BA44.4.C |
| GSM1400879 |
Control, S59.BA44.4.C |
| GSM1400880 |
Control, S13.BA44.4.C |
| GSM1400881 |
Control, S16.BA44.1.C |
| GSM1400882 |
MDD, S63.BA44.4.S |
| GSM1400883 |
MDD, S65.BA44.1.S |
| GSM1400884 |
MDD, S67.BA44.1.S |
| GSM1400885 |
MDD, S68.BA44.1.S |
| GSM1400886 |
MDD, S77.BA44.4.S |
| GSM1400887 |
MDD, S26.BA44.1.S |
| GSM1400888 |
MDD, S28.BA44.1.S |
| GSM1400889 |
MDD, S31.BA44.4.S |
| GSM1400890 |
MDD, S81.BA44.1.S |
| GSM1400891 |
MDD, S84.BA44.1.S |
| GSM1400892 |
MDD, S85.BA44.1.S |
| GSM1400893 |
MDD, S38.BA44.1.S |
| GSM1400894 |
MDD, S87.BA44.1.S |
| GSM1400895 |
MDD, S89.BA44.1.S |
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| Relations |
| BioProject |
PRJNA248913 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58105_RAW.tar |
56.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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