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Series GSE58105 Query DataSets for GSE58105
Status Public on May 30, 2014
Title Prefrontal cortex microRNA expression profiling in major depression
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To gain insight into the potential role of miRNA in major depressive disorder, in this study we assessed global expression of miRNAs in the ventrolateral prefrontal cortex (PFC) of depressed individuals in comparison to psychiatrically healthy controls. Our screening pointed to miR-1202, a miRNA specific to primates and enriched in the human brain. We validated our findings using quantitative real-time PCR and identified target genes of the differentially expressed miRNAs using bioinformatics analysis. MiR-1202 interacts with and regulates the expression of the metabotropic glutamate receptor 4 (GRM4) gene, and it responds to antidepressant treatment. These results suggest that miR-1202 is associated with the pathophysiology of depression, and is a potential target for novel antidepressant treatments.
Overall design We assessed the global expression pattern of miRNAs in the ventrolateral PFC of depressed individuals (n = 14) in comparison with psychiatrically healthy controls (n = 11) using the Agilent human miRNA microarrays. Statistical analysis after multiple test correction identified miR-1202 as the most significantly dysregulated miRNA, with levels down-regulated in depressed brains. We validated the microarray miR-1202 findings by qRT-PCR using TaqMan probes, and found consistent results across the two expression analysis methods.
Contributor(s) Lopez JP, Lim R, Pavlidis P, Turecki G
Citation(s) 24908571
Submission date May 29, 2014
Last update date Aug 13, 2014
Contact name Paul Pavlidis
Organization name University of British Columbia
Department Centre for High-Throughput Biology / Psychiatry
Street address 177 Michael Smith Laboratories 2185 East Mall
City Vancouver
State/province British Columbia
ZIP/Postal code V6T 1Z4
Country Canada
Platforms (1)
GPL18743 Agilent-021827 Human miRNA Microarray [miRNA_107_Sep09]
Samples (25)
GSM1400871 Control, S54.BA44.4.C
GSM1400872 Control, S1.BA44.1.C
GSM1400873 Control, S3.BA44.1.C
BioProject PRJNA248913

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58105_RAW.tar 56.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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