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Series GSE27272 Query DataSets for GSE27272
Status Public on Apr 01, 2011
Title Comprehensive Study of Tobacco Smoke-Related Transcriptome Alterations in Maternal and Fetal Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Maternal smoking has a severe negative effect on all stages of pregnancy that in consequence impairs fetal growth and development. Tobacco smoke-related defects are well established at the clinical level; however, little is known about molecular mechanisms underlying these pathological conditions. We thus employed a genomic approach to determine transcriptome alterations induced by maternal smoking in pregnancy. We assayed gene expression profiles in peripheral blood (M) leukocytes and placentas (PL) of pregnant smokers and those without significant exposure, and in cord blood (D) leukocytes of their babies. Comparative analyses defined significant deregulation of 193 genes in M cells, 329 genes in placentas, and 49 genes in D cells of smokers. These genes were mainly involved in xenobiotic metabolism, oxidative stress, inflammation, immunity, hematopoiesis, trophoblast differentiation, and vascularization. Functional annotation of the deregulated genes outlined processes and pathways affected by tobacco smoke. In smoker newborns, we identified several deregulated pathways associated with autoimmune diseases. The study demonstrates a limited ability of placenta to modulate toxic effects of maternal tobacco use at the gene expression level.
Overall design Blood and placental samples were obtained from 91 women who gave birth to a baby in the Ceske Budejovice Hospital from November 2008 to March 2009. The study was approved by the Local Institutional Review Board. All participants provided written informed consent and completed an extensive questionnaire on pregnancy history (medication, diseases), delivery course, newborn characteristics and life style (smoking, diet, alcohol drinking, area of residency). Only subjects who underwent vaginal delivery were included in the study. Based on smoking history, the women were divided into two groups, “smokers” (N=20) and “non-smokers” (N=52), while passive smokers (N=19) were excluded from the study. Smoking status was further confirmed by detection of excessive plasma levels of cotinine (the major nicotine metabolite) in maternal blood.
Contributor(s) Votavova H, Merkerova Dostalova M, Fejglova K, Vasikova A, Krejcik Z, Tabashidze N, Veleminsky M Jr, Pastorkova A, Topinka J, Sram RJ, Brdicka R
Citation(s) 21803418
Submission date Feb 13, 2011
Last update date Mar 20, 2017
Contact name Hana Bruchova
Phone +420221977306
Fax +420221977371
Organization name Institute of Hematology and Transfusion
Department Molecular Genetics
Lab Genomics
Street address U nemocnice 1
City Prague 2
ZIP/Postal code 128 20
Country Czech Republic
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (183)
GSM674181 non-smoker 302M
GSM674182 smoker 303M
GSM674183 non-smoker 305M
BioProject PRJNA137135

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27272_RAW.tar 3.9 Mb (http)(custom) TAR
GSE27272_non-normalized_cord_blood.txt.gz 7.9 Mb (ftp)(http) TXT
GSE27272_non-normalized_maternal_blood.txt.gz 8.1 Mb (ftp)(http) TXT
GSE27272_non-normalized_placenta.txt.gz 6.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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