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| Status |
Public on Mar 03, 2020 |
| Title |
Next generation sequencing facilitates quantitative analysis of retinal transcriptomes under normal light and high-intensity light condition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Photoreceptor cell degeneration is a major cause of blindness and a considerable health burden during aging but effective therapeutic or preventive strategies have not so far become commercially available. Here we show in mouse models that signaling through the tyrosine kinase receptor KIT protects photoreceptor cells against both light-induced and inherited retinal degeneration. Upon light damage, photoreceptor cells upregulate Kit ligand (KITL) and activate KIT signaling, which in turn induces nuclear accumulation of the transcription factor NRF2 and stimulates the expression of the antioxidant gene Hmox1. Conversely, a viable Kit mutation promotes light-induced photoreceptor damage, which is reversed by experimental expression of Hmox1. Furthermore, overexpression of KITL from a viral AAV8 vector prevents photoreceptor cell death and partially restores retinal function after light damage or in genetic models of human retinitis pigmentosa. Hence, application of KITL may provide a novel therapeutic avenue for prevention or treatment of retinal degeneration
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| Overall design |
Retinal mRNA profiles of 2-month old BALB/c mice exposed to normal light or high-intensity light were generated by deep sequencing, in quintupled, using Illumina Novaseq
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| Contributor(s) |
Li H, Hou L |
| Citation(s) |
32242818 |
| |
| Submission date |
Mar 02, 2020 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Huirong Li |
| E-mail(s) |
huirong2009@aliyun.com
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| Organization name |
Wenzhou medical university
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| Department |
School of Ophthalmology and Optometry and Eye Hospital
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| Lab |
Laboratory of Developmental Cell Biology and Disease
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| Street address |
NO.268, Xueyuan road, Lucheng district
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| City |
Wenzhou city |
| State/province |
Zhejiang province |
| ZIP/Postal code |
325027 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA609651 |
| SRA |
SRP251240 |
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