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| Status |
Public on Aug 24, 2018 |
| Title |
Expression data (U133 Plus 2.0) from fibroblast like synoviocytes from patients with rheumatoid arthritis (RA-FLS) stimulated by TL1A |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
TNF-like ligand 1A (TL1A) is a member of TNF receptor superfamily and involved in the pathogenesis of autoimmune diseases including rheumatoid arthritis (RA) by inducing apoptosis via intracellular death domain or promoting inflammation through the activation of NFκB by binding to its specific receptor death receptor 3 (DR3). Meanwhile, decoy receptor 3 (DcR3) competitively binds soluble TL1A in addition to Fas-ligand (FasL) and LIGHT and inhibits the signaling of TL1A via DR3. Therefore, TL1A-DcR3/DR3 signaling may be involved in the pathogenesis of RA by modulating apoptosis and proliferation of RA-FLS.
We hypothesized that TL1A regulates the gene expression in RA-FLS. We used to search for genes in which expression in RA-FLS is regulated by TL1A.
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| Overall design |
RA-FLS were obtained from 4 RA patients (sample1-4). Each sample was incubated with either 1.0 μg/ml recombinant human TL1A protein or phosphate buffered saline (PBS) diluted with serum-free Opti-MEM medium as non-stimulated control for 12 hours at 37°C with 5% CO2. Gene expression in RA-FLS stimulated by TL1A was compared with that of their respective non-stimulated controls.
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| Contributor(s) |
Maeda T, Miura Y, Fukuda K, Hayashi S, Kurosaka M, Kuroda R |
| Citation(s) |
31258900 |
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| Submission date |
Aug 23, 2018 |
| Last update date |
Jul 09, 2019 |
| Contact name |
Koji Fukuda |
| Organization name |
Kobe University Graduate School of Medicine
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| Department |
Department of Orthopaedic Surgery
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| Street address |
7-5-1 Kusunoki, Chuo
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| City |
Kobe |
| ZIP/Postal code |
650-0017 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA487531 |
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