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Series GSE113798 Query DataSets for GSE113798
Status Public on Jul 10, 2018
Title m6A/m-Seq of human B-lymphocyte cell lines from healthy controls and major depressive disorder patients
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary N6-Methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the human stress response are currently unknown. Here, we provide m6A/m-Seq of immortalied cell lines derived from B lymphocytes from male healthy donors or male donors diagnosed with major depressive disorder (MDD), harvested 1 h after treatment with 100 nM cortisol or mock treatment. PolyA-RNA-fragments of each sample was processed both as m6A/m-sample (RNA immunoprecipiation RIP with an m6A and m6Am antibody) and RNA-input sample.
 
Overall design 3 biological replicates of cells from healthy male donors and 3 biological replicates of cells from male donors diagnosed with major depressive disorder each split in two treatment samples: mock treatment (ethanol only) or 100 nM cortisol in ethanol for 1 h creating 4 groups with each 3 biological replicates: healthy-mock: H_x_M, healthy-cortisol: H_x_C, MDD-mock: M_x_M and MDD-cortisol: M_x_C. Each samples was processed as m6A/m-immunoprecipitation-sample ("m6A") or as input RNA samples ("RNA"). Additionally, 1 RNA sample (equimolar mixed from all samples) was processed as IgG control.
 
Contributor(s) Engel M, Chen A
Citation(s) 30048615
Submission date Apr 27, 2018
Last update date May 23, 2021
Contact name Mareen Engel
E-mail(s) mareen_engel@psych.mpg.de
Organization name Max-Planck-Institute of Psychiatry
Department Stress Neurobiology and Neurogenetics
Street address Kraepelinstr. 2-10
City Munich
ZIP/Postal code 80804
Country Germany
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (25)
GSM3119575 input sample of healthy donor H_1 treated with Mock
GSM3119576 input sample of healthy donor H_1 treated with Cort
GSM3119577 input sample of MDD donor M_1 treated with Mock
This SubSeries is part of SuperSeries:
GSE113801 The role of m6A/m-RNA methylation in stress response regulation
Relations
BioProject PRJNA454062
SRA SRP143700

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Supplementary file Size Download File type/resource
GSE113798_processed_data_files.tar.gz 834.9 Mb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record
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