|
| Status |
Public on Aug 31, 2012 |
| Title |
PSU_ChipSeq_G1E-ER4_GATA1_(SC-265)_diffProtD_7hr_Timecourse |
| Sample type |
SRA |
| |
|
| Source name |
G1E-ER4
|
| Organism |
Mus musculus |
| Characteristics |
lab: PSU-m
lab description: Hardison - Penn State University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: G1E-ER4
cell organism: mouse
cell description: Gata1 restored erythroid cells, differentiation induced by estradiol (E2)
cell sex: M
antibody: GATA1_(SC-265)
antibody antibodydescription: rat monoclonal antibody raised against mouse recombinant GATA1; recommended for detection of GATA1 of mouse, rat and human origin. Antibody Target: GATA1
antibody targetdescription: GATA1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-265
treatment: diffProtD_7hr
treatment description: 7 hours differentiation of G1E-ER4 cells with 10 nM beta-estradiol (Hardison)
labexpid: Timecourse
age: E0
age description: Embryonic day 0 (stem cell)
control: Input
control description: Control signal which may be subtracted from experimental raw signal before peaks are called.
controlid: wgEncodeEM003207
labexpid: Timecourse
replicate: 1
strain: 129
strain description: Strain 129, has widely available embryonic stem cells
|
| Biomaterial provider |
Mitch Weiss lab
|
| Treatment protocol |
diffProtD_7hr
|
| Growth protocol |
G1E_ER4_E2_Hardison_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodePsuTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodePsuTfbs
|
| |
|
| Submission date |
Aug 31, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (3) |
| GSE36029 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/PSU |
| GSE49847 |
A comparative encyclopedia of DNA elements in the mouse genome |
| GSE51338 |
Dynamic shifts in occupancy by TAL1 are guided by GATA factors and drive large-scale reprogramming of gene expression during hematopoiesis |
|
| Relations |
| SRA |
SRX181742 |
| BioSample |
SAMN01141811 |
| Named Annotation |
GSM995442_mm9_wgEncodePsuTfbsG1eer4Gata1aME0S129InputDiffd7hSigRep1.bigWig |
