NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM994848 Query DataSets for GSM994848
Status Public on Nov 01, 2012
Title Inflammatory macrophages donor2
Sample type RNA
 
Source name Ovarian cancer donor 2
Organism Homo sapiens
Characteristics cell: cells isolated from ascites
cell population: inflammatory macrophages
Extracted molecule total RNA
Extraction protocol RNA was extracted using Rneasy Microquit (Qiagen).
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Genesprings GX 7.3 (Agilent)
Data processing Statistical analysis were performed using GenSpring GX 7.3 (Agilent). Expression data was normalized using the RMA algorithm. Probes with an expression lower than 25 Units were filtered out.
 
Submission date Aug 30, 2012
Last update date Nov 01, 2012
Contact name Maxime Touzot
E-mail(s) mtouzot@hotmail.com
Organization name Institut Curie
Lab U932
Street address 26 rue d'ULM
City PAris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE40484 Human inflammatory dendritic cells induce Th17 differentiation

Data table header descriptions
ID_REF
VALUE log(2) RMA

Data table
ID_REF VALUE
7892501 4.660163
7892502 6.007515
7892503 3.130101
7892504 8.190635
7892505 3.051576
7892506 7.284663
7892507 3.98726
7892508 8.34606
7892509 10.76148
7892510 5.511481
7892511 5.933239
7892512 5.459182
7892513 4.94677
7892514 11.16959
7892515 8.457873
7892516 4.001985
7892517 6.233579
7892518 3.416969
7892519 6.147575
7892520 8.581048

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM994848_IS09_ARN0009_s1h1_A07_hGene.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap