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Sample GSM994846 Query DataSets for GSM994846
Status Public on Nov 01, 2012
Title Inflammatory macrophages donor1
Sample type RNA
 
Source name Ovarian cancer donor 1
Organism Homo sapiens
Characteristics cell: cells isolated from ascites
cell population: inflammatory macrophages
Extracted molecule total RNA
Extraction protocol RNA was extracted using Rneasy Microquit (Qiagen).
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Genesprings GX 7.3 (Agilent)
Data processing Statistical analysis were performed using GenSpring GX 7.3 (Agilent). Expression data was normalized using the RMA algorithm. Probes with an expression lower than 25 Units were filtered out.
 
Submission date Aug 30, 2012
Last update date Nov 01, 2012
Contact name Maxime Touzot
E-mail(s) mtouzot@hotmail.com
Organization name Institut Curie
Lab U932
Street address 26 rue d'ULM
City PAris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE40484 Human inflammatory dendritic cells induce Th17 differentiation

Data table header descriptions
ID_REF
VALUE log(2) RMA

Data table
ID_REF VALUE
7892501 3.577142
7892502 6.143869
7892503 2.402858
7892504 8.019956
7892505 3.945174
7892506 6.276316
7892507 3.812004
7892508 6.871686
7892509 11.06835
7892510 5.348008
7892511 5.62262
7892512 4.444153
7892513 4.092322
7892514 11.07221
7892515 8.943443
7892516 2.886314
7892517 5.473237
7892518 3.123078
7892519 5.665648
7892520 8.335397

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM994846_IS09_ARN0007_s1h1_G05_hGene.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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