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Sample GSM994840 Query DataSets for GSM994840
Status Public on Nov 01, 2012
Title CD14+CD16- monocytes donor1
Sample type RNA
 
Source name Healthy donor1
Organism Homo sapiens
Characteristics cell: primary cells
cell population: CD14+CD16- monocytes
Extracted molecule total RNA
Extraction protocol RNA was extracted using Rneasy Microquit (Qiagen).
Label biotin
Label protocol 100 ng of polysomal-bound RNA were employed to synthesize double-stranded cDNA using 2 successive reverse-transcription reactions according to the std affymetrix protocol
 
Hybridization protocol Labelled DNA was hybrized on the Affymetrix Human Gene 1.1 ST processed on an Affymetrix GeneTitan device.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from Genesprings GX 7.3 (Agilent)
Data processing Statistical analysis were performed using GenSpring GX 7.3 (Agilent). Expression data was normalized using the RMA algorithm. Probes with an expression lower than 25 Units were filtered out.
 
Submission date Aug 30, 2012
Last update date Nov 01, 2012
Contact name Maxime Touzot
E-mail(s) mtouzot@hotmail.com
Organization name Institut Curie
Lab U932
Street address 26 rue d'ULM
City PAris
ZIP/Postal code 75005
Country France
 
Platform ID GPL11532
Series (1)
GSE40484 Human inflammatory dendritic cells induce Th17 differentiation

Data table header descriptions
ID_REF
VALUE log(2) RMA

Data table
ID_REF VALUE
7892501 4.966237
7892502 7.879293
7892503 4.03331
7892504 7.809139
7892505 4.453857
7892506 7.773458
7892507 4.399626
7892508 8.410226
7892509 10.39347
7892510 6.43744
7892511 5.862317
7892512 5.198338
7892513 4.286291
7892514 10.83212
7892515 8.3157
7892516 2.820851
7892517 7.102786
7892518 2.948438
7892519 6.043796
7892520 8.798728

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM994840_IS09_ARN0001_s1h1_A05_hGene.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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