|
Status |
Public on Mar 01, 2019 |
Title |
13904269 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
dimboa2cy3
|
Organism |
Spodoptera frugiperda |
Characteristics |
label: dimboa2cy3 sample/extract: dimboa2 subject: L6 larvae tissue: midgut dvt_stage: 6 rna_number: 10
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy3
|
Label protocol |
cDNA Pronto Promega
|
|
|
Channel 2 |
Source name |
dmso2dimcy5
|
Organism |
Spodoptera frugiperda |
Characteristics |
label: dmso2dimcy5 sample/extract: dmso2dim subject: L6 larvae tissue: midgut dvt_stage: 6 rna_number: 10
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy5
|
Label protocol |
cDNA Pronto Promega
|
|
|
|
Hybridization protocol |
Corning Pronto : 750.0 ng at 42 degree_C during 16 hours
|
Scan protocol |
Scanned on an GenePix 4000B fluorescent scanner. Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
Description |
dimboa2cy3 vs dmso2dimcy5 Date:2012/08/06
|
Data processing |
Unnormalized, log (base 2) transform of the (Ch1/Ch2) ratio of the medians shown in the data table below. Normalized data [i.e., Log (base 2) transform of the (Ch1/Ch2) ratio of the medians;limma package: normalizeWithinArray(loess, background:subtract) normalizeBetweenArrays (quantile); lmFit ;eBayes] linked as supplementary files on the Series record.
|
|
|
Submission date |
Aug 21, 2012 |
Last update date |
Mar 01, 2019 |
Contact name |
frederique hilliou |
E-mail(s) |
hilliou@sophia.inra.fr
|
Phone |
0033(0)492386578
|
Organization name |
INRA
|
Department |
SPE
|
Lab |
insect functional genomic
|
Street address |
400 route des chappes, BP167
|
City |
Sophia Antipolis cedex |
ZIP/Postal code |
06903 |
Country |
France |
|
|
Platform ID |
GPL8717 |
Series (1) |
GSE40262 |
Induction Spodoptera midgut after induction by benzoxazinoids |
|