|
| Status |
Public on Mar 01, 2019 |
| Title |
13904276 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
diboa2cy3
|
| Organism |
Spodoptera frugiperda |
| Characteristics |
label: diboa2cy3
sample/extract: diboa2
subject: L6 larvae
tissue: midgut
dvt_stage: 6
rna_number: 10
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
cDNA Pronto Promega
|
| |
|
| Channel 2 |
| Source name |
dmsodi2cy5
|
| Organism |
Spodoptera frugiperda |
| Characteristics |
label: dmsodi2cy5
sample/extract: dmsodi2
subject: L6 larvae
tissue: midgut
dvt_stage: 6
rna_number: 10
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
cDNA Pronto Promega
|
| |
|
| |
| Hybridization protocol |
Corning Pronto : 750.0 ng at 42 degree_C during 16 hours
|
| Scan protocol |
Scanned on an GenePix 4000B fluorescent scanner. Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
| Description |
diboa2cy3 vs dmsodi2cy5
Date:2012/08/06
|
| Data processing |
Unnormalized, log (base 2) transform of the (Ch1/Ch2) ratio of the medians shown in the data table below. Normalized data [i.e., Log (base 2) transform of the (Ch1/Ch2) ratio of the medians;limma package: normalizeWithinArray(loess, background:subtract) normalizeBetweenArrays (quantile); lmFit ;eBayes] linked as supplementary files on the Series record.
|
| |
|
| Submission date |
Aug 21, 2012 |
| Last update date |
Mar 01, 2019 |
| Contact name |
frederique hilliou |
| E-mail(s) |
hilliou@sophia.inra.fr
|
| Phone |
0033(0)492386578
|
| Organization name |
INRA
|
| Department |
SPE
|
| Lab |
insect functional genomic
|
| Street address |
400 route des chappes, BP167
|
| City |
Sophia Antipolis cedex |
| ZIP/Postal code |
06903 |
| Country |
France |
| |
|
| Platform ID |
GPL8717 |
| Series (1) |
| GSE40262 |
Induction Spodoptera midgut after induction by benzoxazinoids |
|
