|
| Status |
Public on Sep 21, 2012 |
| Title |
Donor 2, GM (Cy3) vs. GM+IL-4 (Cy5) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
primary CD14+ monocytes, GM-CSF + IL-4
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary CD14+ monocytes
treatment: GM-CSF + IL-4
donor: 2
|
| Treatment protocol |
Monocytes were cultured for 6 hours in the presence of select cytokines as described for each sample.
|
| Growth protocol |
Monocytes were isolated from PBMCs using the RosetteSep Human Monocytes Enrichment Kit followed by CD14-positive selection by MACS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed in PBS and then lysed in Trizol. Total mRNA was isolated using a combined Trizol and RNeasy Micro kit protocol.
|
| Label |
Cy5
|
| Label protocol |
RNA was labelled using the Two-Color Low Input Quick Amp Labeling kit from Agilent.
|
| |
|
| Channel 2 |
| Source name |
Primary CD14+ monocytes, GM-CSF
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary CD14+ monocytes
treatment: GM-CSF
donor: 2
|
| Treatment protocol |
Monocytes were cultured for 6 hours in the presence of select cytokines as described for each sample.
|
| Growth protocol |
Monocytes were isolated from PBMCs using the RosetteSep Human Monocytes Enrichment Kit followed by CD14-positive selection by MACS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed in PBS and then lysed in Trizol. Total mRNA was isolated using a combined Trizol and RNeasy Micro kit protocol.
|
| Label |
Cy3
|
| Label protocol |
RNA was labelled using the Two-Color Low Input Quick Amp Labeling kit from Agilent.
|
| |
|
| |
| Hybridization protocol |
As per the Agilent standard protocol.
|
| Scan protocol |
Data were scanned at the Stanford Human Immune Monitoring Core.
|
| Description |
Donor 2
|
| Data processing |
Data were extracted and normalized using Feature Extraction software (Agilent).
|
| |
|
| Submission date |
Aug 17, 2012 |
| Last update date |
Sep 21, 2012 |
| Contact name |
Remi J Creusot |
| E-mail(s) |
rcreusot@stanford.edu
|
| URL |
http://fathmanlab.stanford.edu/people/index.html
|
| Organization name |
Stanford University
|
| Department |
Medicine
|
| Lab |
Fathman
|
| Street address |
269 Campus Dr, CCSR 2240
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE40200 |
Human monocyte-derived DCs: effect of IL-4 superkines |
|
