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Sample GSM988221 Query DataSets for GSM988221
Status Public on Sep 21, 2012
Title Donor 1, GM (Cy3) vs. GM+KFR (Cy5)
Sample type RNA
 
Channel 1
Source name primary CD14+ monocytes, GM-CSF + KFR
Organism Homo sapiens
Characteristics cell type: primary CD14+ monocytes
treatment: GM-CSF + KFR
donor: 1
Treatment protocol Monocytes were cultured for 6 hours in the presence of select cytokines as described for each sample.
Growth protocol Monocytes were isolated from PBMCs using the RosetteSep Human Monocytes Enrichment Kit followed by CD14-positive selection by MACS.
Extracted molecule total RNA
Extraction protocol Cells were washed in PBS and then lysed in Trizol. Total mRNA was isolated using a combined Trizol and RNeasy Micro kit protocol.
Label Cy5
Label protocol RNA was labelled using the Two-Color Low Input Quick Amp Labeling kit from Agilent.
 
Channel 2
Source name Primary CD14+ monocytes, GM-CSF
Organism Homo sapiens
Characteristics cell type: primary CD14+ monocytes
treatment: GM-CSF
donor: 1
Treatment protocol Monocytes were cultured for 6 hours in the presence of select cytokines as described for each sample.
Growth protocol Monocytes were isolated from PBMCs using the RosetteSep Human Monocytes Enrichment Kit followed by CD14-positive selection by MACS.
Extracted molecule total RNA
Extraction protocol Cells were washed in PBS and then lysed in Trizol. Total mRNA was isolated using a combined Trizol and RNeasy Micro kit protocol.
Label Cy3
Label protocol RNA was labelled using the Two-Color Low Input Quick Amp Labeling kit from Agilent.
 
 
Hybridization protocol As per the Agilent standard protocol.
Scan protocol Data were scanned at the Stanford Human Immune Monitoring Core.
Description Donor 1
Data processing Data were extracted and normalized using Feature Extraction software (Agilent).
 
Submission date Aug 17, 2012
Last update date Sep 21, 2012
Contact name Remi J Creusot
E-mail(s) rcreusot@stanford.edu
URL http://fathmanlab.stanford.edu/people/index.html
Organization name Stanford University
Department Medicine
Lab Fathman
Street address 269 Campus Dr, CCSR 2240
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL13607
Series (1)
GSE40200 Human monocyte-derived DCs: effect of IL-4 superkines

Data table header descriptions
ID_REF
VALUE Lowess-normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 3.866984916e-002
2 0.000000000e+000
3 0.000000000e+000
4 -9.113065297e-003
5 2.008043663e-002
6 -6.550738261e-002
7 1.668315459e-001
8 1.109424511e-001
9 -1.625220136e-001
10 -3.066993582e-001
11 1.258866040e+000
12 -3.504733919e-002
13 2.897931468e-001
14 8.484744901e-002
15 0.000000000e+000
16 0.000000000e+000
17 -1.242901102e-001
18 0.000000000e+000
19 -1.873329201e-001
20 2.379297120e-002

Total number of rows: 62976

Table truncated, full table size 1425 Kbytes.




Supplementary file Size Download File type/resource
GSM988221_A1D1.txt.gz 20.7 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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