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Sample GSM984952 Query DataSets for GSM984952
Status Public on Jun 04, 2013
Title CD45- hemogenic precursors FLT3-TKD/MLLAF4 rep1
Sample type RNA
 
Source name CD45- hemogenic precursors FLT3-TKD/MLLAF4 rep1
Organism Homo sapiens
Characteristics cell type: CD45- hemogenic precursors
genotype/variation: FLT3-TKD/MLLAF4
precursor: Yes
Extracted molecule total RNA
Extraction protocol Hemogenic progenitors (CD31+CD45-) and blood cells (CD45+) derived from hESCs were sorted out at day 15 of EB development for gene expression profiling14,33. The following transgenic hESC lines were used: EV, FLT3-TKD, MLLAF4/FLT3-TKD and MLLAF4/FLT3-WT. Cells were collected and stabilized in Prep-Protec solution (Miltenyi Biotech) until RNA extraction. RNA was isolated using the NucleoSpin RNAII kit, and its quality checked in the Agilent 2100 Bioanalyzer platform (Agilent Technologies). For the linear T7-based amplification, 100ng of each total RNA sample was used
Label biotin
Label protocol Labeled samples were hybridized overnight with the Gene Expression Hybridization kit to a Whole Human Genome Microarray (G4112F 45.000 probes corresponding to 32.600 genes Agilent Technologies) following the Manufacturer's instructions
 
Hybridization protocol Standard Agilent hybridization protocol
Scan protocol Standard Agilent scanning protocol
Description replicate 1
Data processing Primary data was processed with GeneSpring 11.0 software (Silicon Genetics, Redwood City, CA). Data postprocessing was performed with in-house developed functions in Matlab
 
Submission date Aug 14, 2012
Last update date Jan 15, 2022
Contact name Marcos J. Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL13607
Series (1)
GSE40103 FLT3 activation cooperates with MLL-AF4 fusion gene to abrogate the hematopoietic specification of human ESCs

Data table header descriptions
ID_REF
VALUE log2 quantile normalized signal intensity

Data table
ID_REF VALUE
4 9.835799
5 2.427147
6 5.075557
7 7.586418
8 8.074408
9 1.833850
10 6.551805
11 2.106703
12 8.920792
13 8.939593
14 7.202644
15 9.231985
16 6.883350
17 6.839066
18 1.673446
19 1.670292
20 2.743793
21 7.472172
24 6.345230
25 6.727673

Total number of rows: 58717

Table truncated, full table size 858 Kbytes.




Supplementary file Size Download File type/resource
GSM984952_CD45-_hemogenic_precursors_FLT3-TKD_MLLAF4-rep1.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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