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Sample GSM984946 Query DataSets for GSM984946
Status Public on Jun 04, 2013
Title CD45- hemogenic precursors EV rep1
Sample type RNA
 
Source name CD45- hemogenic precursors EV rep1
Organism Homo sapiens
Characteristics cell type: CD45- hemogenic precursors
genotype/variation: EV
precursor: Yes
Extracted molecule total RNA
Extraction protocol Hemogenic progenitors (CD31+CD45-) and blood cells (CD45+) derived from hESCs were sorted out at day 15 of EB development for gene expression profiling14,33. The following transgenic hESC lines were used: EV, FLT3-TKD, MLLAF4/FLT3-TKD and MLLAF4/FLT3-WT. Cells were collected and stabilized in Prep-Protec solution (Miltenyi Biotech) until RNA extraction. RNA was isolated using the NucleoSpin RNAII kit, and its quality checked in the Agilent 2100 Bioanalyzer platform (Agilent Technologies). For the linear T7-based amplification, 100ng of each total RNA sample was used
Label biotin
Label protocol Labeled samples were hybridized overnight with the Gene Expression Hybridization kit to a Whole Human Genome Microarray (G4112F 45.000 probes corresponding to 32.600 genes Agilent Technologies) following the Manufacturer's instructions
 
Hybridization protocol Standard Agilent hybridization protocol
Scan protocol Standard Agilent scanning protocol
Description replicate 1
Data processing Primary data was processed with GeneSpring 11.0 software (Silicon Genetics, Redwood City, CA). Data postprocessing was performed with in-house developed functions in Matlab
 
Submission date Aug 14, 2012
Last update date Jan 15, 2022
Contact name Marcos J. Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL13607
Series (1)
GSE40103 FLT3 activation cooperates with MLL-AF4 fusion gene to abrogate the hematopoietic specification of human ESCs

Data table header descriptions
ID_REF
VALUE log2 quantile normalized signal intensity

Data table
ID_REF VALUE
4 10.109133
5 2.858124
6 6.104295
7 7.945117
8 7.256642
9 1.623812
10 7.228342
11 1.802177
12 8.791124
13 9.177703
14 6.895674
15 9.492610
16 6.176688
17 6.474408
18 1.615183
19 1.614233
20 2.649339
21 7.472411
24 6.707903
25 8.816092

Total number of rows: 58717

Table truncated, full table size 858 Kbytes.




Supplementary file Size Download File type/resource
GSM984946_CD45-_hemogenic_precursors_EV-rep1.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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