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Sample GSM983164 Query DataSets for GSM983164
Status Public on Aug 10, 2012
Title Tumor_1586_HCI
Sample type RNA
 
Source name Primary tumor 1586 generated from EF1α-PyMT9C lentivirus, adenocarcinoma histology
Organism Mus musculus
Characteristics tissue type: mouse mammary tumor
lentivirus: EF1α-PyMT9C
primary tumor: N/A
histology: Adenocarcinoma
number of insertion sites: Unknown
combat group: 1
Treatment protocol No treatments
Growth protocol Mammary epithelial cells were infected with specified lentivirus, then transplanted into cleared fat pads of recipient mice. Tumors were harvested at 2 cm and a portion was flash frozen.
Extracted molecule total RNA
Extraction protocol Qiagen Rneasy kit
Label Cy3
Label protocol Agilent One-Color Quick Amp Labeling Protocol
 
Hybridization protocol Agilent One-color Gene Expression Hybridization and Wash Protocol
Scan protocol Microarrays were scanned on an Agilent Technologies G2505C scanner at 5 micron resolution
Data processing Array images were processed in Agilent Feature Extraction software version 10.5.1.1, which produces spatially detrended intensity data
Array data was filtered to remove control features and any non-uniform features or population outlier features. Remaining features were averaged to yield a single value for each unique probe. Finally data were log2 transformed, quantile normalized, and adjusted for batch effects using ComBat.
 
Submission date Aug 09, 2012
Last update date Feb 07, 2013
Contact name Bryan Welm
E-mail(s) Bryan.Welm@hci.utah.edu
Phone 801-587-4633
Organization name University of Utah, Huntsman Cancer Institute
Department Oncological Science
Lab Bryan Welm Lab
Street address 2000 Circle of Hope Drive
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
 
Platform ID GPL7202
Series (1)
GSE40001 Targeting PyMT oncogene to diverse mammary cell populations enhances tumor heterogeneity and generates rare breast cancer subtypes

Data table header descriptions
ID_REF
VALUE Log 2 transformed normalized intensity data

Data table
ID_REF VALUE
A_51_P100021 3.024185841
A_51_P100034 10.64882758
A_51_P100052 2.606908492
A_51_P100084 8.274965282
A_51_P100099 8.002372108
A_51_P100155 10.39335884
A_51_P100174 7.8558039
A_51_P100218 4.702657543
A_51_P100227 9.742698101
A_51_P100238 3.282723754
A_51_P100246 9.303189092
A_51_P100289 10.41392219
A_51_P100327 7.436542664
A_51_P100347 3.241895989
A_51_P100470 6.905300463
A_51_P100537 4.496599462
A_51_P100565 6.536419685
A_51_P100624 3.835548094
A_51_P100625 6.105667601
A_51_P100768 3.685769207

Total number of rows: 13988

Table truncated, full table size 339 Kbytes.




Supplementary file Size Download File type/resource
GSM983164_Tumor_1586_HCI.txt.gz 9.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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