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Sample GSM983141 Query DataSets for GSM983141
Status Public on Aug 10, 2012
Title Tumor_25_HCI
Sample type RNA
Source name Primary tumor 25 generated from EF1α-PyMT10C lentivirus, adenoarcinoma histology
Organism Mus musculus
Characteristics tissue type: mouse mammary tumor
lentivirus: EF1α-PyMT10C
primary tumor: N/A
histology: Adenocarcinoma
number of insertion sites: 1
combat group: 4
Treatment protocol No treatments
Growth protocol Mammary epithelial cells were infected with specified lentivirus, then transplanted into cleared fat pads of recipient mice. Tumors were harvested at 2 cm and a portion was flash frozen.
Extracted molecule total RNA
Extraction protocol Qiagen Rneasy kit
Label Cy3
Label protocol Agilent One-Color Quick Amp Labeling Protocol
Hybridization protocol Agilent One-color Gene Expression Hybridization and Wash Protocol
Scan protocol Microarrays were scanned on an Agilent Technologies G2505C scanner at 5 micron resolution
Data processing Array images were processed in Agilent Feature Extraction software version, which produces spatially detrended intensity data
Array data was filtered to remove control features and any non-uniform features or population outlier features. Remaining features were averaged to yield a single value for each unique probe. Finally data were log2 transformed, quantile normalized, and adjusted for batch effects using ComBat.
Submission date Aug 09, 2012
Last update date Feb 07, 2013
Contact name Bryan Welm
Phone 801-587-4633
Organization name University of Utah, Huntsman Cancer Institute
Department Oncological Science
Lab Bryan Welm Lab
Street address 2000 Circle of Hope Drive
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
Platform ID GPL7202
Series (1)
GSE40001 Targeting PyMT oncogene to diverse mammary cell populations enhances tumor heterogeneity and generates rare breast cancer subtypes

Data table header descriptions
VALUE Log 2 transformed normalized intensity data

Data table
A_51_P100021 4.581100034
A_51_P100034 10.50403036
A_51_P100052 3.576016508
A_51_P100084 7.777497303
A_51_P100099 8.02966271
A_51_P100155 10.77150012
A_51_P100174 8.257468254
A_51_P100218 3.955672749
A_51_P100227 10.38842543
A_51_P100238 4.126568915
A_51_P100246 8.899756101
A_51_P100289 9.232968616
A_51_P100327 6.460307974
A_51_P100347 3.181813132
A_51_P100470 6.699936657
A_51_P100537 5.162977739
A_51_P100565 7.824985362
A_51_P100624 4.975931432
A_51_P100625 6.285150898
A_51_P100768 4.459104161

Total number of rows: 13988

Table truncated, full table size 340 Kbytes.

Supplementary file Size Download File type/resource
GSM983141_Tumor_25_HCI.txt.gz 9.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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