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Sample GSM982323 Query DataSets for GSM982323
Status Public on Jul 05, 2013
Title KELLY, bafilomycin incubation, rep 2
Sample type RNA
 
Source name KELLY, bafilomycin incubation
Organism Homo sapiens
Characteristics cell line: KELLY
Treatment protocol KELLY cells were incubated with a final concentration of 10 µM genistein for 48 h or 1 nM of bafilomycin A1 for 48 h. Genistein and bafilomycin were solved in 1% DMSO; 1% DMSO (final) was used as control.
Growth protocol KELLY cells cultured in RPMI1640 medium
Extracted molecule total RNA
Extraction protocol RNA extraction using Nucleospin-RNA-II (Macherey-Nagel)
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Sense Target Labeling Assay (Affymetrix).
 
Hybridization protocol Samples were hybridized with Genechip Human Gene 1.0 ST Arrays using Affymetrix Hybridization Kit.
Scan protocol Scanning was performed at the microarray core facility at the Charité, Berlin, Germany (http://lfgc.charite.de/).
Description FK_75
Data processing Data were processed using Chipinspector software (Genomatix GmbH). Raw data were normalised on the single probe level and subsequent pairwise comparison analyses of the expression values from the experimental condition versus control conditions were performed based on a single-sided permutational t-test analysis.
ChipInspector carries out significance analysis on the single probe level. Normalized probe set level data not provided.
 
Submission date Aug 08, 2012
Last update date Jul 05, 2013
Contact name Daniela Zaade
E-mail(s) Daniela.Zaade@charite.de, Daniela.Zaade@gmx.de
Phone 0049(0)30450525304
Organization name Charité
Department Center for Cardiovascular Research
Lab AG Heiko Funke-Kaiser
Street address Hessische Straße 3-4
City Berlin
ZIP/Postal code 10115
Country Germany
 
Platform ID GPL10739
Series (2)
GSE39962 Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: Geni_Bafi]
GSE39965 Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses

Supplementary file Size Download File type/resource
GSM982323_FK_75.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data are available on Series record

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