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Sample GSM982318 Query DataSets for GSM982318
Status Public on Jul 05, 2013
Title HEK293T, stable PLZF overexpression, replica 3
Sample type RNA
 
Source name HEK293T, stable PLZF overexpression
Organism Homo sapiens
Characteristics cell line: HEK293T
genetic modification: stable PLZF overexpression
Treatment protocol not applicable
Growth protocol HEK293T cells were cultured in DMEM medium.
Extracted molecule total RNA
Extraction protocol RNA extraction using Nucleospin-RNA-II (Macherey-Nagel)
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Sense Target Labeling Assay (Affymetrix).
 
Hybridization protocol Samples were hybridized with Genechip Human Gene 1.0 ST Arrays using Affymetrix Hybridization Kit.
Scan protocol Scanning was performed at the microarray core facility at the Charité, Berlin, Germany (http://lfgc.charite.de/).
Description FK_44
Data processing Data were processed using Chipinspector software (Genomatix GmbH). Raw data were normalised on the single probe level and subsequent pairwise comparison analyses of the expression values from the experimental condition versus control conditions were performed based on a single-sided permutational t-test analysis.
ChipInspector carries out significance analysis on the single probe level. Normalized probe set level data not provided.
 
Submission date Aug 08, 2012
Last update date Jul 05, 2013
Contact name Daniela Zaade
E-mail(s) Daniela.Zaade@charite.de, Daniela.Zaade@gmx.de
Phone 0049(0)30450525304
Organization name Charité
Department Center for Cardiovascular Research
Lab AG Heiko Funke-Kaiser
Street address Hessische Straße 3-4
City Berlin
ZIP/Postal code 10115
Country Germany
 
Platform ID GPL10739
Series (2)
GSE39961 Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: PLZF_HEK]
GSE39965 Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses

Supplementary file Size Download File type/resource
GSM982318_FK_44.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data are available on Series record

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