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Sample GSM973449 Query DataSets for GSM973449
Status Public on Jul 26, 2012
Title +/+ hippocampus 1
Sample type RNA
Source name hippocampus, transgenic
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: transgenic
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
VALUE RMA log2 transformed signal values.

Data table
10338001 9.7258962
10338003 8.34199464
10338004 7.625233954
10338017 11.27896627
10338025 6.549696891
10338026 11.57260076
10338029 7.508626724
10338035 6.687452672
10338036 7.446562274
10338037 2.073820233
10338041 9.334588386
10338042 8.503046552
10338044 10.28576054
10338047 3.557214009
10338056 1.944408705
10338059 11.23967609
10338060 1.985704433
10338064 2.326393532
10338065 3.372185985
10338066 2.002479304

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.

Supplementary file Size Download File type/resource
GSM973449_E10R025f01.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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