GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM973448 Query DataSets for GSM973448
Status Public on Jul 26, 2012
Title wt hippocampus 3
Sample type RNA
Source name hippocampus, wild type
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: wild type
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
VALUE RMA log2 transformed signal values.

Data table
10338001 9.581889747
10338003 8.192519682
10338004 7.675604253
10338017 11.13981991
10338025 6.573063933
10338026 11.30470496
10338029 7.341007257
10338035 6.552295601
10338036 7.35009939
10338037 2.079005975
10338041 9.137121794
10338042 8.322441963
10338044 10.12254331
10338047 3.522419893
10338056 1.958063131
10338059 11.05493664
10338060 2.048832534
10338064 2.218641695
10338065 3.28912622
10338066 2.103406996

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.

Supplementary file Size Download File type/resource
GSM973448_E10R025e03.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap