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Sample GSM973447 Query DataSets for GSM973447
Status Public on Jul 26, 2012
Title wt hippocampus 2
Sample type RNA
 
Source name hippocampus, wild type
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: wild type
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
 
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
 
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
E-mail(s) direnberger@bio.lmu.de
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
ID_REF
VALUE RMA log2 transformed signal values.

Data table
ID_REF VALUE
10338001 9.697770345
10338003 8.176487341
10338004 7.719019115
10338017 11.33256249
10338025 6.575067221
10338026 11.64965587
10338029 7.435203523
10338035 6.579674964
10338036 7.574479999
10338037 2.092298788
10338041 9.2644426
10338042 8.500359905
10338044 10.32631712
10338047 3.556159613
10338056 1.943133676
10338059 11.17997749
10338060 1.990907677
10338064 2.28760527
10338065 3.338324571
10338066 2.027709644

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.




Supplementary file Size Download File type/resource
GSM973447_E10R025e02.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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