GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM973446 Query DataSets for GSM973446
Status Public on Jul 26, 2012
Title wt hippocampus 1
Sample type RNA
Source name hippocampus, wild type
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: wild type
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
VALUE RMA log2 transformed signal values.

Data table
10338001 9.653540167
10338003 8.228279984
10338004 7.590145379
10338017 11.19858623
10338025 6.50935381
10338026 11.53117186
10338029 7.410815954
10338035 6.583558195
10338036 7.379534326
10338037 2.123576811
10338041 9.096309298
10338042 8.407671407
10338044 10.21942266
10338047 3.618837696
10338056 1.987753791
10338059 11.12720765
10338060 2.020875517
10338064 2.314220865
10338065 3.396844497
10338066 2.025737561

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.

Supplementary file Size Download File type/resource
GSM973446_E10R025e01.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap