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Sample GSM973439 Query DataSets for GSM973439
Status Public on Jul 26, 2012
Title +/+ skeletal muscle 3
Sample type RNA
Source name skeletal muscle, transgenic
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: transgenic
tissue: skeletal muscle
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
VALUE RMA log2 transformed signal values.

Data table
10338001 9.408057949
10338003 8.065367663
10338004 7.168311086
10338017 10.87892454
10338025 6.552084986
10338026 11.23627735
10338029 7.114887534
10338035 6.263697367
10338036 6.955091899
10338037 1.698947422
10338041 8.731743291
10338042 8.198228928
10338044 9.953837046
10338047 3.412497022
10338056 1.522804028
10338059 10.93075951
10338060 1.593994295
10338064 1.88420325
10338065 3.34285338
10338066 1.625457537

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.

Supplementary file Size Download File type/resource
GSM973439_E10R025b03.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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