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Sample GSM973435 Query DataSets for GSM973435
Status Public on Jul 26, 2012
Title wt skeletal muscle 2
Sample type RNA
Source name skeletal muscle, wild type
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: wild type
tissue: skeletal muscle
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
VALUE RMA log2 transformed signal values.

Data table
10338001 9.510325063
10338003 8.123940317
10338004 7.391088892
10338017 10.99334178
10338025 6.485288348
10338026 11.28952327
10338029 7.269014439
10338035 6.36317283
10338036 7.157013384
10338037 1.724440727
10338041 8.787598974
10338042 8.003107462
10338044 10.04018011
10338047 3.399294162
10338056 1.540429064
10338059 11.00817648
10338060 1.599693893
10338064 1.900041761
10338065 3.314753072
10338066 1.646337798

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.

Supplementary file Size Download File type/resource
GSM973435_E10R025a02.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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