|
Status |
Public on Oct 04, 2012 |
Title |
GATA1-A |
Sample type |
SRA |
|
|
Source name |
Human primary adult proerythroblasts (ProEs)
|
Organism |
Homo sapiens |
Characteristics |
tissue: CD34+ HSPC-derived proerythroblasts developmental stage: adult bone marrow chip antibody: GATA1 vendor: Abcam catalog#: ab11852
|
Growth protocol |
Primary maturing fetal or adult erythroblasts were generated ex vivo using a serum-free two-phase liquid culture system.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
For ChIP-seq analysis using the Illumin HiSeq2000, 10-20 ng of ChIP DNA was processed for library generation using the ChIP-seq Sample Preparation Kit (Illumina) following the manufacturer’s protocol. Raw ChIP-seq data were processed using the Illumina software pipeline. Only ChIP-seq reads that aligned to exactly one location in the reference human genome (UCSC, hg18) were retained for downstream data analysis.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Sequencing reads were aligned to human genome assembly hg18 (NCBI version 36) using Illumina software pipeline. Only ChIP-seq reads that aligned to exactly one location in the reference human genome (UCSC, hg18) were retained for downstream data analysis. Peak detection was performed with the Model-based Analysis of ChIP-Seq (MACS) algorithm (http://liulab.dfci.harvard.edu/MACS/). Genome_build: hg18 Supplementary_files_format_and_content: Mapped read bed file, wig file, and peak bed file
|
|
|
Submission date |
Jul 19, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Jian Xu |
E-mail(s) |
Jian.Xu@stjude.org
|
Phone |
9015955208
|
Organization name |
St. Jude Children's Research Hospital
|
Department |
Pathology
|
Street address |
262 Danny Thomas Place, MS 345
|
City |
Memphis |
State/province |
Tennessee |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (2) |
GSE36985 |
Comparative profiling of chromatin state maps and transcription factor occupancy during human fetal and adult erythropoiesis |
GSE36994 |
Comparative profiling of human fetal and adult erythropoiesis |
|
Relations |
SRA |
SRX160844 |
BioSample |
SAMN01091708 |