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Sample GSM967934 Query DataSets for GSM967934
Status Public on Jan 20, 2015
Title Brain-Alzheimer's disease PSEN1 mutation carrier patient-3
Sample type RNA
Source name Frozen brain tissue: posterior cingulate at thalamus level
Organism Homo sapiens
Characteristics gender: Male
age at death: 54
post-mortem interval (hours): 7:30
apoe genotype: 3/3
psen1 mutation: V89L
neuropathological data (braak stage): A3, B3, C3 + CAA moderate
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen brain tissue (posterior cingulate area) using the RNeasy Lipid Tissue Mini Kit (Qiagen)
Label Biotin
Label protocol Labeled ssDNA was prepared according to the standard Affymetrix Terminal labeling Kit protocol.
Hybridization protocol Following fragmentation, 5 ug of ssDNA were hybridized for 16 hr at 45C following Affymetrix standard procedures
Scan protocol GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program, scanner PlateScanner M10)
Description Gene expression data of final stages of Alzheimer's disease
Data processing Raw data was background adjusted, normalized and summarized by Robust Multi-array Analysis (RMA) using the Affy package (version 1.30.0) from Bioconductor on R language (version 2.13.2).
Submission date Jul 17, 2012
Last update date Jan 20, 2015
Contact name Anna Antonell
Organization name Hospital Clínic Barcelona
Department Unitat d'Alzheimer i altres transtorns cognitius
Lab Malalties neurodegeneratives
Street address C/Casanova, 143
City Barcelona
ZIP/Postal code 08036
Country Spain
Platform ID GPL11532
Series (1)
GSE39420 Gene expression profile of sporadic and PSEN1 early-onset Alzheimer’s Disease

Data table header descriptions
VALUE log2 RMA normalized signal intensity

Data table
7892501 2.458
7892502 4.480
7892503 2.513
7892504 8.654
7892505 2.656
7892506 2.867
7892507 3.581
7892508 6.569
7892509 9.283
7892510 3.071
7892511 4.530
7892512 5.540
7892513 3.158
7892514 9.656
7892515 8.793
7892516 2.120
7892517 6.487
7892518 3.746
7892519 3.352
7892520 7.893

Total number of rows: 33297

Table truncated, full table size 456 Kbytes.

Supplementary file Size Download File type/resource
GSM967934_P3.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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