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Sample GSM967923 Query DataSets for GSM967923
Status Public on Jan 20, 2015
Title Brain-control 6
Sample type RNA
 
Source name Frozen brain tissue: posterior cingulate at thalamus level
Organism Homo sapiens
Characteristics gender: Male
age at death: 49
post-mortem interval (hours): 7:35
apoe genotype: 3/3
psen1 mutation: None
neuropathological data (braak stage): No neuropathological findings
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen brain tissue (posterior cingulate area) using the RNeasy Lipid Tissue Mini Kit (Qiagen)
Label Biotin
Label protocol Labeled ssDNA was prepared according to the standard Affymetrix Terminal labeling Kit protocol.
 
Hybridization protocol Following fragmentation, 5 ug of ssDNA were hybridized for 16 hr at 45C following Affymetrix standard procedures
Scan protocol GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.1.0.1228, scanner PlateScanner M10)
Description Gene expression data of final stages of Alzheimer's disease
Data processing Raw data was background adjusted, normalized and summarized by Robust Multi-array Analysis (RMA) using the Affy package (version 1.30.0) from Bioconductor on R language (version 2.13.2).
 
Submission date Jul 17, 2012
Last update date Jan 20, 2015
Contact name Anna Antonell
E-mail(s) antonell@clinic.ub.es
Organization name Hospital Clínic Barcelona
Department Unitat d'Alzheimer i altres transtorns cognitius
Lab Malalties neurodegeneratives
Street address C/Casanova, 143
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL11532
Series (1)
GSE39420 Gene expression profile of sporadic and PSEN1 early-onset Alzheimer’s Disease

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal intensity

Data table
ID_REF VALUE
7892501 2.234
7892502 3.513
7892503 2.648
7892504 8.426
7892505 2.362
7892506 2.736
7892507 3.319
7892508 6.090
7892509 10.384
7892510 3.254
7892511 3.782
7892512 4.672
7892513 2.706
7892514 9.452
7892515 9.072
7892516 2.221
7892517 6.624
7892518 2.867
7892519 3.138
7892520 7.624

Total number of rows: 33297

Table truncated, full table size 456 Kbytes.




Supplementary file Size Download File type/resource
GSM967923_C6.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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