|
| Status |
Public on Nov 07, 2012 |
| Title |
B cells, CD19_D |
| Sample type |
genomic |
| |
|
| Source name |
B cells, healthy
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: B cells from whole blood
gender: male
disease state: healthy
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Mononuclear cells and B cells were selected by Lymphoprep or CD19 microbeads protocols, and total DNA was isolated by standard procedures.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol.
|
| |
|
| Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 BeadChips using the standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using High Scan SQ using the standard recommended Illumina scanner setting.
|
| Description |
CD19_D
|
| Data processing |
Genome Studio V2011.2. The methylation score of each CpG is represented as an average beta value (AVG_Beta).
|
| |
|
| Submission date |
Jul 05, 2012 |
| Last update date |
Nov 30, 2012 |
| Contact name |
Manel Esteller |
| Organization name |
IDIBELL
|
| Department |
PEBC
|
| Lab |
Cancer Epigenetics
|
| Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
| City |
L'Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE39141 |
Genome-wide DNA methylation profiling predicts relapse in childhood B-cell acute lymphoblastic leukemia |
|
