|
| Status |
Public on Apr 05, 2013 |
| Title |
271_4 |
| Sample type |
RNA |
| |
|
| Source name |
hBMSCs, old donor, passage 4
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: bone marrow-derived mesenchymal stem cells
passage: 4
age: 75 years
subject id: 271
|
| Treatment protocol |
NA
|
| Growth protocol |
The cells were cultured in minimum essential alpha-medium (αMEM) supplemented with 20mM HEPES, 10% heat-inactivated fetal bovine serum (FBS), 2mM L-glutamine and 100 units/ml penicillin and 100 μg/ml streptomycin (all from Gibco, Paisley, UK). Same serum lot was used throughout the study. The medium was renewed twice a week and the cells were harvested when 70-80% confluent and plated further at density of 1000 cells/cm2. Cells were cultured until senescence was reached.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen AllPrep DNA/RNA Mini Kit (Cat. no. 80204, Qiagen, CA, USA) and a Qiagen supplementary protocol “Purification of total RNA containing miRNA from animal cells using the RNeasy Plus Mini Kit (cat. no. 74134).
|
| Label |
Cy5
|
| Label protocol |
Cy3 and Cy5, following the manufacturer's recommendations.
|
| |
|
| Hybridization protocol |
According to the manufacturer's recommendations.
|
| Scan protocol |
According to the manufacturer's recommendations.
|
| Description |
271_4_Cy5
271-4_271-8_US23502401_252800414962_S01_GE2_1100_Jul11_1_4
This Sample represents one channel of a dual-channel array.
|
| Data processing |
Data was imported into R and analyzed with the BioConductor limma package. Data was quantile normalized.
|
| |
|
| Submission date |
Jun 29, 2012 |
| Last update date |
Apr 05, 2013 |
| Contact name |
Dario Greco |
| E-mail(s) |
dario.greco@tuni.fi
|
| Organization name |
Tampere University
|
| Department |
Faculty of Medicine and Health Technology
|
| Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
|
| Street address |
Arvo ylpön Katu 34
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE39035 |
Human bone marrow mesenchymal stem cells, young vs. old donors, early vs. late passages |
|
