|
Status |
Public on Apr 05, 2013 |
Title |
271_8 |
Sample type |
RNA |
|
|
Source name |
hBMSCs, old donor, passage 8
|
Organism |
Homo sapiens |
Characteristics |
cell type: bone marrow-derived mesenchymal stem cells passage: 8 age: 75 years subject id: 271
|
Treatment protocol |
NA
|
Growth protocol |
The cells were cultured in minimum essential alpha-medium (αMEM) supplemented with 20mM HEPES, 10% heat-inactivated fetal bovine serum (FBS), 2mM L-glutamine and 100 units/ml penicillin and 100 μg/ml streptomycin (all from Gibco, Paisley, UK). Same serum lot was used throughout the study. The medium was renewed twice a week and the cells were harvested when 70-80% confluent and plated further at density of 1000 cells/cm2. Cells were cultured until senescence was reached.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Qiagen AllPrep DNA/RNA Mini Kit (Cat. no. 80204, Qiagen, CA, USA) and a Qiagen supplementary protocol “Purification of total RNA containing miRNA from animal cells using the RNeasy Plus Mini Kit (cat. no. 74134).
|
Label |
Cy3
|
Label protocol |
Cy3 and Cy5, following the manufacturer's recommendations.
|
|
|
Hybridization protocol |
According to the manufacturer's recommendations.
|
Scan protocol |
According to the manufacturer's recommendations.
|
Description |
271_8_Cy3 271-4_271-8_US23502401_252800414962_S01_GE2_1100_Jul11_1_4 This Sample represents one channel of a dual-channel array.
|
Data processing |
Data was imported into R and analyzed with the BioConductor limma package. Data was quantile normalized.
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|
|
Submission date |
Jun 29, 2012 |
Last update date |
Apr 05, 2013 |
Contact name |
Dario Greco |
E-mail(s) |
dario.greco@tuni.fi
|
Organization name |
Tampere University
|
Department |
Faculty of Medicine and Health Technology
|
Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
|
Street address |
Arvo ylpön Katu 34
|
City |
Tampere |
ZIP/Postal code |
33520 |
Country |
Finland |
|
|
Platform ID |
GPL13607 |
Series (1) |
GSE39035 |
Human bone marrow mesenchymal stem cells, young vs. old donors, early vs. late passages |
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