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Sample GSM954293 Query DataSets for GSM954293
Status Public on Apr 05, 2013
Title 271_8
Sample type RNA
 
Source name hBMSCs, old donor, passage 8
Organism Homo sapiens
Characteristics cell type: bone marrow-derived mesenchymal stem cells
passage: 8
age: 75 years
subject id: 271
Treatment protocol NA
Growth protocol The cells were cultured in minimum essential alpha-medium (αMEM) supplemented with 20mM HEPES, 10% heat-inactivated fetal bovine serum (FBS), 2mM L-glutamine and 100 units/ml penicillin and 100 μg/ml streptomycin (all from Gibco, Paisley, UK). Same serum lot was used throughout the study. The medium was renewed twice a week and the cells were harvested when 70-80% confluent and plated further at density of 1000 cells/cm2. Cells were cultured until senescence was reached.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen AllPrep DNA/RNA Mini Kit (Cat. no. 80204, Qiagen, CA, USA) and a Qiagen supplementary protocol “Purification of total RNA containing miRNA from animal cells using the RNeasy Plus Mini Kit (cat. no. 74134).
Label Cy3
Label protocol Cy3 and Cy5, following the manufacturer's recommendations.
 
Hybridization protocol According to the manufacturer's recommendations.
Scan protocol According to the manufacturer's recommendations.
Description 271_8_Cy3
271-4_271-8_US23502401_252800414962_S01_GE2_1100_Jul11_1_4
This Sample represents one channel of a dual-channel array.
Data processing Data was imported into R and analyzed with the BioConductor limma package. Data was quantile normalized.
 
Submission date Jun 29, 2012
Last update date Apr 05, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL13607
Series (1)
GSE39035 Human bone marrow mesenchymal stem cells, young vs. old donors, early vs. late passages

Data table header descriptions
ID_REF
VALUE Log2 quantile-normalized median foreground value

Data table
ID_REF VALUE
1 15.5124763
2 6.116638031
3 6.080370384
4 7.959914905
5 7.955266116
6 6.3858288
7 11.71304435
8 10.01473401
9 6.132050575
10 6.415024542
11 6.056883443
12 9.027518023
13 10.25817376
14 8.733289701
15 10.90049605
16 6.132050575
17 7.484707894
18 5.793450449
19 6.004316903
20 9.641213234

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM954293_271-4_271-8_US23502401_252800414962_S01_GE2_1100_Jul11_1_4.txt.gz 6.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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