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Sample GSM954292 Query DataSets for GSM954292
Status Public on Apr 05, 2013
Title 194_8
Sample type RNA
Source name hBMSCs, old donor, passage 8
Organism Homo sapiens
Characteristics cell type: bone marrow-derived mesenchymal stem cells
passage: 8
age: 82 years
subject id: 194
Treatment protocol NA
Growth protocol The cells were cultured in minimum essential alpha-medium (αMEM) supplemented with 20mM HEPES, 10% heat-inactivated fetal bovine serum (FBS), 2mM L-glutamine and 100 units/ml penicillin and 100 μg/ml streptomycin (all from Gibco, Paisley, UK). Same serum lot was used throughout the study. The medium was renewed twice a week and the cells were harvested when 70-80% confluent and plated further at density of 1000 cells/cm2. Cells were cultured until senescence was reached.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen AllPrep DNA/RNA Mini Kit (Cat. no. 80204, Qiagen, CA, USA) and a Qiagen supplementary protocol “Purification of total RNA containing miRNA from animal cells using the RNeasy Plus Mini Kit (cat. no. 74134).
Label Cy5
Label protocol Cy3 and Cy5, following the manufacturer's recommendations.
Hybridization protocol According to the manufacturer's recommendations.
Scan protocol According to the manufacturer's recommendations.
Description 194_8_Cy5
This Sample represents one channel of a dual-channel array.
Data processing Data was imported into R and analyzed with the BioConductor limma package. Data was quantile normalized.
Submission date Jun 29, 2012
Last update date Apr 05, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL13607
Series (1)
GSE39035 Human bone marrow mesenchymal stem cells, young vs. old donors, early vs. late passages

Data table header descriptions
VALUE Log2 quantile-normalized median foreground value

Data table
1 15.90325475
2 6.122637002
3 6.175129494
4 8.177965948
5 7.047947251
6 6.318407186
7 11.38301215
8 10.15057924
9 6.15754334
10 6.617151739
11 5.968441998
12 9.043903317
13 10.40229903
14 8.691037376
15 7.288088697
16 6.0950826
17 7.32716683
18 6.0950826
19 6.175129494
20 10.03129872

Total number of rows: 62976

Table truncated, full table size 1086 Kbytes.

Supplementary file Size Download File type/resource
GSM954292_194-4_194-8_US23502401_252800414962_S01_GE2_1100_Jul11_1_3.txt.gz 6.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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