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Sample GSM954283 Query DataSets for GSM954283
Status Public on Apr 05, 2013
Title 091_4
Sample type RNA
 
Source name hBMSCs, young donor, passage 4
Organism Homo sapiens
Characteristics cell type: bone marrow-derived mesenchymal stem cells
passage: 4
age: 23 years
subject id: 91
Treatment protocol NA
Growth protocol The cells were cultured in minimum essential alpha-medium (αMEM) supplemented with 20mM HEPES, 10% heat-inactivated fetal bovine serum (FBS), 2mM L-glutamine and 100 units/ml penicillin and 100 μg/ml streptomycin (all from Gibco, Paisley, UK). Same serum lot was used throughout the study. The medium was renewed twice a week and the cells were harvested when 70-80% confluent and plated further at density of 1000 cells/cm2. Cells were cultured until senescence was reached.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen AllPrep DNA/RNA Mini Kit (Cat. no. 80204, Qiagen, CA, USA) and a Qiagen supplementary protocol “Purification of total RNA containing miRNA from animal cells using the RNeasy Plus Mini Kit (cat. no. 74134).
Label Cy3
Label protocol Cy3 and Cy5, following the manufacturer's recommendations.
 
Hybridization protocol According to the manufacturer's recommendations.
Scan protocol According to the manufacturer's recommendations.
Description 091_4_Cy3
091-4_091-8_US23502401_252800414962_S01_GE2_1100_Jul11_2_3
This Sample represents one channel of a dual-channel array.
Data processing Data was imported into R and analyzed with the BioConductor limma package. Data was quantile normalized.
 
Submission date Jun 29, 2012
Last update date Apr 05, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL13607
Series (1)
GSE39035 Human bone marrow mesenchymal stem cells, young vs. old donors, early vs. late passages

Data table header descriptions
ID_REF
VALUE Log2 quantile-normalized median foreground value

Data table
ID_REF VALUE
1 15.34091996
2 6.204674969
3 6.043331771
4 8.002995503
5 7.235037572
6 6.398320842
7 11.51136892
8 9.741855224
9 6.204674969
10 6.509579877
11 6.191396453
12 9.137990388
13 10.44876749
14 8.419240535
15 8.687418883
16 6.204674969
17 7.086695872
18 6.159347695
19 6.136573984
20 9.752561548

Total number of rows: 62976

Table truncated, full table size 1088 Kbytes.




Supplementary file Size Download File type/resource
GSM954283_091-4_091-8_US23502401_252800414962_S01_GE2_1100_Jul11_2_3.txt.gz 5.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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