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Sample GSM9532 Query DataSets for GSM9532
Status Public on Oct 29, 2003
Title 24 hr A-chip 3-13-03
Sample type RNA
Source name IB3-1 bronchial epithelial cell line
Organism Homo sapiens
Extracted molecule total RNA
Description IB3-1 (deltaF508/W1282X) bronchial epithelial cells were cultured in T75 flasks with gentamicin-free LHC-8 medium. After reaching 80% confluence cells were treated with 1 mM PBA for 24 hr. A T75 flask of confluent IB3-1 cells was rinsed twice with ice cold Hank’s buffer then scraped into 3ml of ice cold TRIzol (Gibco BRL) then rinsed with 3 ml ice cold TRIzol and the mRNA was isolated according to the TRIzol protocol. Array images were assessed by eye to confirm scanner alignment and the absence of significant bubbles or scratches. Chip scanning and expression analysis was performed using Microarray Analysis Suite 5.0 software and scaled to an average probe set intensity of 150.
Submission date Sep 03, 2003
Last update date Oct 28, 2005
Contact name Jerry M. Wright
Phone 410-614-5010
Organization name Johns Hopkins Medical Institutions
Department Physiology
Street address 725 N. Wolfe St
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platform ID GPL96
Series (1)
GSE620 Gene expression profile analysis of 4-phenylbutyrate treatment of IB3-1 bronchial epithelial cell line

Data table header descriptions
VALUE chip scaled to 150
ABS_CALL Present/Marginal/Absent

Data table
AFFX-BioB-5_at 152.6 P
AFFX-BioB-M_at 269.2 P
AFFX-BioB-3_at 142.1 P
AFFX-BioC-5_at 407.2 P
AFFX-BioC-3_at 318.1 P
AFFX-BioDn-5_at 373.2 P
AFFX-BioDn-3_at 2437.6 P
AFFX-CreX-5_at 4015.9 P
AFFX-CreX-3_at 6263.9 P
AFFX-DapX-5_at 4.6 A
AFFX-DapX-M_at 14.8 A
AFFX-DapX-3_at 2.6 A
AFFX-LysX-5_at 6.7 A
AFFX-LysX-M_at 17.1 A
AFFX-LysX-3_at 4.8 A
AFFX-PheX-5_at 1.2 A
AFFX-PheX-M_at 2.1 A
AFFX-PheX-3_at 20.6 A
AFFX-ThrX-5_at 3.4 A
AFFX-ThrX-M_at 14.6 A

Total number of rows: 22283

Table truncated, full table size 395 Kbytes.

Supplementary file Size Download File type/resource
GSM9532.CEL.gz 3.4 Mb (ftp)(http) CEL

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