NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM94893 Query DataSets for GSM94893
Status Public on Sep 20, 2006
Title LOF_stage12_replicate4
Sample type RNA
 
Channel 1
Source name stage12 embryo wt
Organism Drosophila melanogaster
Characteristics Oregon R wildtype embryos
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage12 embryo mutant
Organism Drosophila melanogaster
Characteristics gcm mutant embryos automated sorted
Extracted molecule polyA RNA
Label Cy5
 
 
Description gcmN7-4 mutant flies were balanced with either Kr-GFP (for stages 10 to 13) or Ubi-GFP (for stages 14 to 16) balancer chromosomes. Eggs were collected as described above, transferred into PBS buffer supplemented with 0.3% Tween 20 and homozygous mutant embryos were automatically sorted with the CopasTM Select embryo sorter by their lack of GFP-expression. For wt control we used OregonR wildtype embryos that were treated the same way like the mutant embryos.Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide
Data processing Genepix 5.0/MChips software
 
Submission date Feb 02, 2006
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in �m of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R? (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
Sum of Medians
Sum of Means
F Pixels
B Pixels
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R? (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 Sum of Medians Sum of Means F Pixels B Pixels VALUE
1 01_61_03_12 5280 5980 90 18440 17781 10697 406 617 837 100 100 0 65535 52280 20286 0 1072 5779 98 94 59 0.275 0.332 0.305 0.290 1.600 0.324 0.818 18034 65535 17375 52280 83569 69655 52 313 -1.862
2 01_61_05_12 5400 5990 100 603 628 560 460 637 827 10 1 0 0 341 514 0 1045 5792 0 0 0 100.000.000 0.493 0.752 0.671 2.622 0.321 0.557 143 0 168 341 143 509 80 310 16.610
3 01_61_07_12 5540 6000 100 357 393 382 421 500 469 15 0 0 0 54 222 0 78 242 5 5 0 100.000.000 -0.519 0.305 0.293 1.498 5.944 0.056 -64 0 -28 54 -64 26 80 259 16.610
4 01_61_09_12 5670 6000 100 480 617 580 320 407 389 36 16 0 0 38 132 0 101 272 5 2 0 100.000.000 7.816 3.610 5.527 7.815 0.013 0.001 160 0 297 38 160 335 80 282 16.610
5 01_61_11_12 5790 6020 70 1312 1427 722 315 427 408 81 68 0 11933 11138 2965 0 183 449 100 100 0 0.084 0.100 0.105 0.086 2.314 0.079 0.313 997 11933 1112 11138 12930 12250 32 138 -3.581
6 01_61_13_12 5920 6010 80 2081 2133 1172 310 420 431 90 71 0 35517 35154 19183 0 190 607 100 100 5 0.050 0.052 0.056 0.050 2.000 0.047 0.682 1771 35517 1823 35154 37288 36977 52 229 -4.326
7 01_61_15_12 6060 6000 70 4419 5145 3607 408 476 444 90 87 0 31715 35735 23671 0 384 905 100 100 25 0.126 0.133 0.133 0.124 1.734 0.125 0.865 4011 31715 4737 35735 35726 40472 32 201 -2.983
8 01_61_01_24 6200 6020 80 7716 8601 4951 467 505 452 98 98 0 65535 54792 18905 74 457 721 100 100 65 0.111 0.149 0.138 0.134 1.682 0.154 0.786 7249 65461 8134 54718 72710 62852 52 222 -3.175
9 01_61_03_24 6340 6010 100 175 335 396 327 404 410 18 5 0 0 145 288 0 203 531 8 3 0 100.000.000 0.055 1.086 1.265 4.263 13.985 0.000 -152 0 8 145 -152 153 80 294 16.610
10 01_61_05_24 6470 6010 100 276 365 371 258 401 441 22 1 0 0 170 361 0 140 361 18 6 0 100.000.000 0.629 0.712 1.440 8.953 6.089 0.004 18 0 107 170 18 277 80 311 16.610
11 01_61_07_24 6610 6010 100 244 328 366 316 440 463 12 1 0 0 102 284 0 175 387 11 6 0 100.000.000 0.118 0.857 1.126 8.675 18.678 0.001 -72 0 12 102 -72 114 80 282 16.610
12 01_61_09_24 6740 6020 100 218 317 365 231 392 442 13 2 0 0 269 429 0 199 363 28 15 0 100.000.000 0.320 0.443 0.527 2.980 0.171 0.009 -13 0 86 269 -13 355 80 242 16.610
13 01_61_11_24 6870 6020 100 312 438 444 237 377 428 28 8 0 288 570 657 0 200 340 47 33 0 0.260 0.353 0.521 0.641 4.800 0.009 0.000 75 288 201 570 363 771 80 262 -1.941
14 01_61_13_24 7010 6020 100 263 359 352 234 347 394 23 6 0 0 285 704 0 119 290 28 11 0 100.000.000 0.439 0.791 0.627 4.860 238.395 0.000 29 0 125 285 29 410 80 340 16.610
15 01_61_15_24 7170 6000 50 1341 1190 436 412 433 378 75 66 0 626 666 542 0 146 315 75 50 0 1.484 1.168 1.606 0.937 3.171 0.799 0.002 929 626 778 666 1555 1444 12 81 0.570
16 01_62_01_12 7270 6020 100 194 300 348 357 425 401 13 2 0 0 148 316 0 166 363 15 7 0 100.000.000 -0.385 0.490 0.443 6.368 0.093 0.002 -163 0 -57 148 -163 91 80 314 16.610
17 01_62_03_12 7410 6030 100 334 430 415 207 371 441 27 8 0 0 216 377 0 180 407 22 8 0 100.000.000 1.032 0.500 0.719 3.508 0.497 0.010 127 0 223 216 127 439 80 256 16.610
18 01_62_05_12 7540 6030 100 373 440 410 268 401 464 30 5 0 0 286 391 0 213 365 33 16 0 100.000.000 0.601 0.719 0.584 2.679 2.032 0.005 105 0 172 286 105 458 80 268 16.610
19 01_62_07_12 7670 6030 100 292 404 417 388 457 473 8 2 0 0 217 393 0 194 407 21 5 0 100.000.000 0.074 0.945 0.770 2.733 0.025 0.000 -96 0 16 217 -96 233 80 319 16.610
20 01_62_09_12 7810 6030 100 358 410 399 328 406 388 23 5 0 0 207 403 0 200 400 21 8 0 100.000.000 0.396 0.557 0.788 4.277 13.035 0.000 30 0 82 207 30 289 80 274 16.610

Total number of rows: 47616

Table truncated, full table size 8492 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap