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Sample GSM947607 Query DataSets for GSM947607
Status Public on Aug 21, 2012
Title Vector-3
Sample type RNA
 
Source name UKE1 cell line
Organism Homo sapiens
Characteristics treatment: GFP
Treatment protocol UKE1 cells were transduced with lentivirus containing shRNA for ASXL1 or vector control in a pLKO.1-puromycin vector followed by puromycin selection for at least 2 weeks
Growth protocol UKE1 cells were grown in RPMI-1640 medium supplemented with 10% FBS + 1mM hydrocortisone + 10% horse serum
Extracted molecule total RNA
Extraction protocol RNA was extracted using TRIZOL according to manufacturers guidelines
Label biotin
Label protocol Please refer to Affymetrix 3' IVT Express Kit Documentaion (http://www.affymetrix.com/estore/browse/products.jsp?categoryIdClicked=&productId=131415#1_3)
 
Hybridization protocol Please see AffymetrixExpression Analysis Technical Manual (http://www.affymetrix.com/estore/browse/products.jsp?categoryIdClicked=&productId=131415#1_3)
Scan protocol Please see AffymetrixExpression Analysis Technical Manual (http://www.affymetrix.com/estore/browse/products.jsp?categoryIdClicked=&productId=131415#1_3)
Description GFP control
Data processing RMA : log2 transform : Quantile Normalization : Median Polish using Partek Genomics Suite Version 6.5
 
Submission date Jun 13, 2012
Last update date Aug 21, 2012
Contact name Omar Abdel-Wahab
E-mail(s) abdelwao@mskcc.org
Organization name Memorial Sloan-Kettering Cancer Center
Department HOPP
Lab Dr. Ross Levine
Street address 1275 York Ave.
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL571
Series (1)
GSE38692 ASXL1 Knock Down in Normal CD34+ Cord Blood and UKE1 Cell Lines

Data table header descriptions
ID_REF
VALUE log2 expression value

Data table
ID_REF VALUE
1007_s_at 4.90031
1053_at 7.78995
117_at 4.48613
121_at 6.77887
1255_g_at 2.26039
1294_at 7.86199
1316_at 5.2751
1320_at 3.03278
1405_i_at 9.61471
1431_at 5.9039
1438_at 4.48889
1487_at 7.53307
1494_f_at 4.15372
1598_g_at 5.85179
160020_at 6.46265
1729_at 6.62293
1773_at 4.96649
177_at 3.33255
179_at 7.6216
1861_at 5.19158

Total number of rows: 22215

Table truncated, full table size 407 Kbytes.




Supplementary file Size Download File type/resource
GSM947607_LS2_UKE1_EV2_HG-U133A_2_2.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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