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Sample GSM947605 Query DataSets for GSM947605
Status Public on Aug 21, 2012
Title Vector-1
Sample type RNA
Source name UKE1 cell line
Organism Homo sapiens
Characteristics treatment: GFP
Treatment protocol UKE1 cells were transduced with lentivirus containing shRNA for ASXL1 or vector control in a pLKO.1-puromycin vector followed by puromycin selection for at least 2 weeks
Growth protocol UKE1 cells were grown in RPMI-1640 medium supplemented with 10% FBS + 1mM hydrocortisone + 10% horse serum
Extracted molecule total RNA
Extraction protocol RNA was extracted using TRIZOL according to manufacturers guidelines
Label biotin
Label protocol Please refer to Affymetrix 3' IVT Express Kit Documentaion (
Hybridization protocol Please see AffymetrixExpression Analysis Technical Manual (
Scan protocol Please see AffymetrixExpression Analysis Technical Manual (
Description GFP control
Data processing RMA : log2 transform : Quantile Normalization : Median Polish using Partek Genomics Suite Version 6.5
Submission date Jun 13, 2012
Last update date Aug 21, 2012
Contact name Omar Abdel-Wahab
Organization name Memorial Sloan-Kettering Cancer Center
Department HOPP
Lab Dr. Ross Levine
Street address 1275 York Ave.
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platform ID GPL571
Series (1)
GSE38692 ASXL1 Knock Down in Normal CD34+ Cord Blood and UKE1 Cell Lines

Data table header descriptions
VALUE log2 expression value

Data table
1007_s_at 5.17923
1053_at 7.8127
117_at 4.54353
121_at 6.68849
1255_g_at 2.21566
1294_at 7.98579
1316_at 5.30705
1320_at 3.26221
1405_i_at 9.19847
1431_at 5.77238
1438_at 4.21771
1487_at 7.60303
1494_f_at 4.29381
1598_g_at 5.96298
160020_at 6.57974
1729_at 6.60939
1773_at 5.19298
177_at 3.38367
179_at 7.82904
1861_at 5.3262

Total number of rows: 22215

Table truncated, full table size 407 Kbytes.

Supplementary file Size Download File type/resource
GSM947605_LS1_UKE1_EV1_HG-U133A_2.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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