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Sample GSM942310 Query DataSets for GSM942310
Status Public on Aug 31, 2012
Title Big_Wekiva_1_17 (Quantile)
Sample type RNA
 
Source name whole brain female
Organism Micropterus salmoides
Characteristics tissue: whole brain
location: Big Wekiva, Santa Fe
Extracted molecule total RNA
Extraction protocol STAT60 protocol for total RNA
Label Cy3
Label protocol Agilent one color protocol for Cy3
 
Hybridization protocol Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
Scan protocol Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software (v9.5) formed a composite of the two scans and calculated parameters for Extended Dynamic Range.
Data processing Normalized signal intensity provided here is Quantile Normalization. Data were also normalized using LOESS for the manuscript.
 
Submission date Jun 04, 2012
Last update date Aug 31, 2012
Contact name Christopher Joseph Martyniuk
E-mail(s) CMARTYN@UFL.EDU
Organization name UF
Department Physiological Sciences
Street address CEHT Mowry Road
City Gainesville
State/province FL
ZIP/Postal code 32611
Country USA
 
Platform ID GPL15650
Series (2)
GSE38458 Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury (field study)
GSE38474 Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
1 13.3772
2 1.994756
3 1.95487
4 8.800025
5 5.383769
6 6.603856
7 7.399909
8 1.844663
9 5.7675
10 8.683899
11 5.357922
12 1.760453
13 8.37428
14 7.149647
15 8.800864
16 13.63095
17 10.48792
18 5.029057
19 2.692946
20 8.642712

Total number of rows: 15744

Table truncated, full table size 218 Kbytes.




Supplementary file Size Download File type/resource
GSM942310_US83800208_252031010010_S01_GE1-v5_10_Apr08_1_1.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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