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Sample GSM942288 Query DataSets for GSM942288
Status Public on Aug 31, 2012
Title Welaka_2 (Loess)
Sample type RNA
 
Source name whole brain female
Organism Micropterus salmoides
Characteristics tissue: whole brain
location: St. John's Rivers Florida
Extracted molecule total RNA
Extraction protocol STAT60 protocol for total RNA
Label Cy3
Label protocol Agilent one color protocol for Cy3
 
Hybridization protocol Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
Scan protocol Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software (v9.5) formed a composite of the two scans and calculated parameters for Extended Dynamic Range.
Data processing Normalized signal intensity provided here is LOESS Normalization. Data were also normalized using Quantile for the manuscript.
 
Submission date Jun 04, 2012
Last update date Aug 31, 2012
Contact name Christopher Joseph Martyniuk
E-mail(s) CMARTYN@UFL.EDU
Organization name UF
Department Physiological Sciences
Street address CEHT Mowry Road
City Gainesville
State/province FL
ZIP/Postal code 32611
Country USA
 
Platform ID GPL15650
Series (2)
GSE38458 Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury (field study)
GSE38474 Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury

Data table header descriptions
ID_REF
VALUE loess normalized

Data table
ID_REF VALUE
1 13.34032
2 1.719983
3 2.778073
4 8.552393
5 5.955367
6 6.492482
7 8.346727
8 1.494253
9 6.463247
10 8.887586
11 5.856326
12 1.613114
13 9.027978
14 9.31587
15 8.924356
16 14.16114
17 10.21098
18 4.304053
19 2.989192
20 9.829508

Total number of rows: 15744

Table truncated, full table size 217 Kbytes.




Supplementary file Size Download File type/resource
GSM942288_US83800208_252031010011_S01_GE1-v5_10_Apr08_2_2.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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