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Sample GSM942270 Query DataSets for GSM942270
Status Public on Aug 31, 2012
Title MeHg1 (Loess)
Sample type RNA
 
Source name female hypothalamus
Organism Micropterus salmoides
Characteristics tissue: hypothalamus
treatment: Hg injection 2.5 µg/g body weight
Extracted molecule total RNA
Extraction protocol STAT60 protocol for total RNA
Label Cy3
Label protocol Agilent one color protocol for Cy3
 
Hybridization protocol Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
Scan protocol Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software (v9.5) formed a composite of the two scans and calculated parameters for Extended Dynamic Range.
Data processing Normalized signal intensity provided here is LOESS Normalization. Data were also normalized using Quantile for the manuscript.
 
Submission date Jun 04, 2012
Last update date Aug 31, 2012
Contact name Christopher Joseph Martyniuk
E-mail(s) CMARTYN@UFL.EDU
Organization name UF
Department Physiological Sciences
Street address CEHT Mowry Road
City Gainesville
State/province FL
ZIP/Postal code 32611
Country USA
 
Platform ID GPL15650
Series (2)
GSE38456 Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury (lab study)
GSE38474 Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury

Data table header descriptions
ID_REF
VALUE loess normalized

Data table
ID_REF VALUE
1 13.66018
2 2.540008
3 2.516217
4 9.063847
5 4.939612
6 6.810225
7 7.629898
8 2.449227
9 6.122175
10 9.263108
11 4.378332
12 2.399244
13 9.367332
14 6.793784
15 9.594125
16 14.85348
17 11.07435
18 6.373394
19 3.169207
20 9.64532

Total number of rows: 15744

Table truncated, full table size 218 Kbytes.




Supplementary file Size Download File type/resource
GSM942270_US83800208_252031010009_S01_GE1-v5_10_Apr08_2_1.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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