|
Status |
Public on Aug 31, 2012 |
Title |
Control_2 (Loess) |
Sample type |
RNA |
|
|
Source name |
female hypothalamus
|
Organism |
Micropterus salmoides |
Characteristics |
tissue: hypothalamus treatment: none
|
Extracted molecule |
total RNA |
Extraction protocol |
STAT60 protocol for total RNA
|
Label |
Cy3
|
Label protocol |
Agilent one color protocol for Cy3
|
|
|
Hybridization protocol |
Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
|
Scan protocol |
Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software (v9.5) formed a composite of the two scans and calculated parameters for Extended Dynamic Range.
|
Data processing |
Normalized signal intensity provided here is LOESS Normalization. Data were also normalized using Quantile for the manuscript.
|
|
|
Submission date |
Jun 04, 2012 |
Last update date |
Aug 31, 2012 |
Contact name |
Christopher Joseph Martyniuk |
E-mail(s) |
CMARTYN@UFL.EDU
|
Organization name |
UF
|
Department |
Physiological Sciences
|
Street address |
CEHT Mowry Road
|
City |
Gainesville |
State/province |
FL |
ZIP/Postal code |
32611 |
Country |
USA |
|
|
Platform ID |
GPL15650 |
Series (2) |
GSE38456 |
Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury (lab study) |
GSE38474 |
Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury |
|