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Sample GSM942217 Query DataSets for GSM942217
Status Public on Jul 31, 2012
Title TK2 KO skel muscle, biological rep3
Sample type RNA
 
Source name Hind limb skeletal muscle from TK2 KO 11 days-old mice
Organism Mus musculus
Characteristics strain: C57BL/6
age: 11 days-old
tissue: skeletal muscle
genotype: TK2 knockout, TK2-/-
Growth protocol Official Swedish regulations for use and care of laboratory animals were followed throughout and the experimental protocol was approved by the local ethical commitee (S104-09, S135-11)
Extracted molecule total RNA
Extraction protocol Muscle tissues were harvested immediatelly after we have sacrificed the mice. Total RNA was extracted using Rneasy kit (QIAGEN), according to manufacturer's instructions.
Label biotin
Label protocol Labelling was done using Ambion WT Expression kit (Life Technologies), according to manufacturer's instructions.
 
Hybridization protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Scan protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Description Gene expression data from skeletal muscle of 11 days-old TK2 knockout mouse
Data processing Affymetrix Expression Console Software; Summarization: PLIER, Normalization: Global Median
 
Submission date Jun 04, 2012
Last update date Jul 31, 2012
Contact name João Amorim Paredes
E-mail(s) joao.paredes@ki.se
Organization name Karolinska Institute
Department Laboratory Medicine
Lab Anna Karlsson
Street address Karolinska Universitetssjukhuset Huddinge, F68
City Huddinge
State/province Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platform ID GPL11533
Series (1)
GSE38449 Gene expression analysis in skeletal muscle and heart of 11 days-old TK2 knockout mice

Data table header descriptions
ID_REF
VALUE PLIER normalized signal intensity

Data table
ID_REF VALUE
10338001 1420.63
10338002 39.06
10338003 372.58
10338004 55.3
10338005 3.27
10338006 4.08
10338007 4.87
10338008 7.76
10338009 97.32
10338010 2.44
10338011 18.95
10338012 2.76
10338013 1.69
10338014 2.27
10338015 1.69
10338016 57.51
10338017 2897.58
10338018 26.47
10338019 11.21
10338020 53.63

Total number of rows: 35556

Table truncated, full table size 524 Kbytes.




Supplementary file Size Download File type/resource
GSM942217_JP06_TK2_3_SM_F07.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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