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Sample GSM942212 Query DataSets for GSM942212
Status Public on Jul 31, 2012
Title wt skel muscle, biological rep1
Sample type RNA
Source name Hind limb skeletal muscle from wt 11 days-old mice
Organism Mus musculus
Characteristics strain: C57BL/6
age: 11 days-old
tissue: skeletal muscle
genotype: wild-type, TK2+/+
Growth protocol Official Swedish regulations for use and care of laboratory animals were followed throughout and the experimental protocol was approved by the local ethical commitee (S104-09, S135-11)
Extracted molecule total RNA
Extraction protocol Muscle tissues were harvested immediatelly after we have sacrificed the mice. Total RNA was extracted using Rneasy kit (QIAGEN), according to manufacturer's instructions.
Label biotin
Label protocol Labelling was done using Ambion WT Expression kit (Life Technologies), according to manufacturer's instructions.
Hybridization protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Scan protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Description Gene expression data from skeletal muscle of 11 days-old wild-type mouse
Data processing Affymetrix Expression Console Software; Summarization: PLIER, Normalization: Global Median
Submission date Jun 04, 2012
Last update date Jul 31, 2012
Contact name João Amorim Paredes
Organization name Karolinska Institute
Department Laboratory Medicine
Lab Anna Karlsson
Street address Karolinska Universitetssjukhuset Huddinge, F68
City Huddinge
State/province Stockholm
ZIP/Postal code 14186
Country Sweden
Platform ID GPL11533
Series (1)
GSE38449 Gene expression analysis in skeletal muscle and heart of 11 days-old TK2 knockout mice

Data table header descriptions
VALUE PLIER normalized signal intensity

Data table
10338001 1178.89
10338002 42.71
10338003 319.7
10338004 58.23
10338005 3.43
10338006 4.49
10338007 5.52
10338008 10.16
10338009 101.59
10338010 3.14
10338011 21.36
10338012 3.6
10338013 2.25
10338014 2.93
10338015 2.04
10338016 61.66
10338017 2517.03
10338018 28.96
10338019 12.34
10338020 59.33

Total number of rows: 35556

Table truncated, full table size 526 Kbytes.

Supplementary file Size Download File type/resource
GSM942212_JP01_WT1_SM_B07.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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