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Sample GSM940225 Query DataSets for GSM940225
Status Public on Aug 24, 2012
Title Pediatric Astrocytoma IV (glioblastoma) A4-1983
Sample type RNA
 
Source name Pediatric Astrocytoma Tumor Sample 13
Organism Homo sapiens
Characteristics tumor grade: IV (glioblastoma)
tissue: Pediatric Astrocytoma Tumor
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from flash-frozen pediatric astrocytoma samples. Total cellular RNA was extracted using Qiagen's RNA isolation kit according to manufacturer's protocol, and genomic DNA contamination was removed by an on-column Dnase digestion step. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer
Label Cy3
Label protocol standard Agilent protocol
 
Hybridization protocol standard Agilent protocol
Scan protocol standard Agilent protocol
Data processing Cy3 median signal intensities were recovered from Agilent Feature Extraction software output, converted to Log2 scale and quantile normalized between arrays, and duplicated probe signals were merged by taking their median values (see series normalized data file).
 
Submission date May 30, 2012
Last update date Aug 24, 2012
Contact name Jane Engler
E-mail(s) jane.engler@ucsf.edu
Organization name UCSF
Street address 1450 3rd Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL4133
Series (1)
GSE38330 Gene Expression Profiling of Human Pediatric Astrocytomas

Data table header descriptions
ID_REF
VALUE Agilentn Feature Extraction gProcessedSignal

Data table
ID_REF VALUE
1 1.98E+05
2 5.16E+00
3 5.16E+00
4 5.17E+00
5 5.17E+00
6 5.17E+00
7 5.18E+00
8 5.18E+00
9 5.18E+00
10 5.18E+00
11 5.19E+00
12 5.17E+01
13 1.15E+02
14 5.12E+02
15 1.97E+01
16 9.72E+03
17 1.54E+03
18 4.93E+02
19 3.28E+04
20 5.04E+01

Total number of rows: 45015

Table truncated, full table size 648 Kbytes.




Supplementary file Size Download File type/resource
GSM940225_251485036075_S01_GE1-v5_95_Feb07_1_1.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data are available on Series record
Processed data provided as supplementary file
Processed data included within Sample table

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