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Sample GSM937055 Query DataSets for GSM937055
Status Public on Jan 16, 2013
Title vulvar normal tissue 1102-0370 expression
Sample type RNA
Source name normal tissue
Organism Homo sapiens
Characteristics differentiation: normal tissue
individual: 1102-0370
tissue: normal vulva
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent. Using the Illumina TotalPrep RNA Amplification Kit, 500ng were reversely transcribed, amplified and Biotin-16-UTP-labeled. The amount and quality of labeled CDNA were measured using both a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer.
Label biotin
Label protocol Using the Illumina TotalPrep RNA Amplification Kit, 500ng were reversely transcribed, amplified and Biotin-16-UTP-labeled.
Hybridization protocol 750 ng of biotin-labeled cRNA was hibridized to the HumanHT-12 v.4 Illumina Sentrix BeadCHip according to the manufacturer's instructions.
Scan protocol Standard Illumina scanning protocol
Data processing Bead summary data was imported into GenomeStudio to remove control probes and to produce a text file containing the signal and detection p-values per probe for all samples, as well as imputing missing data using the k-nearest neighbor algorithm (Tech note: Inputing HumanHT-12 Expression BeadChip Data; Illumina, Inc.). The text file was imported into J-Express Pro 2011, and signal intensity values were quantile normalized (Bolstad et al. 2003) and log transformed (base 2). Significance Analysis of Microarrays (SAM) was used to look for differentially expressed genes. To obtain manageable datasets, differentially expressed genes were defined by a fold change ≥2 and q-value = 0.
Submission date May 24, 2012
Last update date Jan 16, 2013
Contact name Francesca Micci
Organization name Oslo Universitetssykehus
Department Institute for medical informatics
Lab cancer cytogenetics
Street address Ullernchausseen 70
City oslo
ZIP/Postal code 0310
Country Norway
Platform ID GPL10558
Series (2)
GSE38228 Expression analysis of 13 squamous cell carcinomas arising in the vulva
GSE38230 SCC of the vulva

Data table header descriptions
VALUE quantile normalized, log2 transformed, 5 missing probes imputed. Sample “03-4” not used.

Data table
ILMN_1762337 6.517686367
ILMN_2055271 6.299316883
ILMN_1736007 6.116173744
ILMN_2383229 6.09098959
ILMN_1806310 6.023684502
ILMN_1779670 5.614187241
ILMN_1653355 6.159006119
ILMN_1717783 5.579732895
ILMN_1705025 6.320367813
ILMN_1814316 6.183635235
ILMN_2359168 5.661193371
ILMN_1731507 5.346782208
ILMN_1787689 5.513566971
ILMN_3241953 6.816075325
ILMN_1745607 8.855031967
ILMN_2136495 11.69224262
ILMN_1668111 6.081682205
ILMN_2295559 6.287271023
ILMN_1735045 7.295712471
ILMN_1680754 6.219957352

Total number of rows: 47323

Table truncated, full table size 1150 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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